Drying Buffer for Polymer Dye Antibody Conjugates
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Solution Overview
Problem
Existing drying technologies fail to prevent non-specific interactions and aggregation of polymer dye antibody conjugates when dried together, leading to false positive populations and reduced resolution in flow cytometry analysis.
Innovation Solution
A novel buffer composition comprising a water-soluble monomer, a protein stabilizer, a carbohydrate stabilizer, and a zwitterionic surfactant is used to dry a plurality of dye conjugates, reducing aggregation and non-specific binding during drying and reconstitution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If prior drying technology using Reagent Buffer is used to dry down polymer dye antibody conjugates, then drying capability is achieved, but aggregation and non-specific interactions occur
Solution Approach 1:
A novel buffer composition is introduced as an intermediary substance between the polymer dye antibody conjugates during drying. This buffer contains specific components that mediate the drying process to prevent aggregation and non-specific interactions while maintaining the conjugates' functionality. The buffer acts as a protective medium that enables drying without the harmful effects observed with prior Reagent Buffer technology.
2Stability of the object's composition
If polymer dye antibody conjugates are dried together in a single tube, then storage stability is improved, but aggregation occurs leading to false positive populations
Solution Approach 1:
The buffer composition parameters are specifically optimized to prevent aggregation during drying. By adjusting the chemical parameters of the buffer (including specific concentrations of stabilizing agents and pH optimization), the drying process is modified to maintain conjugate separation and prevent aggregation, thereby preserving flow cytometry resolution while achieving storage stability.
3Device complexity
If classical drying technology is used, then simplicity is maintained, but it cannot prevent interaction between polymer dye conjugates
Solution Approach 1:
The buffer composition is formulated as a composite material containing multiple stabilizing components working together. This composite buffer system combines different stabilizing agents and buffering components to provide comprehensive protection against aggregation and non-specific interactions during drying, maintaining both simplicity of use and high reliability of conjugate stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel buffer composition effectively decreases aggregation and non-specific interaction of fluorescent dye conjugates, maintaining their functionality and physical properties, thereby improving flow cytometry resolution and reducing false positive populations.
Implementation Method 1
a zwitterionic surfactant is used to dry a plurality of dye conjugates, reducing aggregation and non-specific binding during drying and reconstitution
Data Source
AI summary
A novel dry down buffer is provided for use in drying a plurality of fluorescent dye conjugates on a substrate for use in flow cytometry. The aqueous buffer comprises a water-soluble monomer; a protein stabilizer; a carbohydrate stabilizer; and a zwitterionic surfactant. When mixed with a multi-color panel comprising fluorescent polymer dye conjugates, dried on a substrate, and reconstituted with a biological sample, the buffer provides decreased aggregation of fluorescent polymer dye conjugates, and decreased non-specific binding of monocytes and granulocytes, when compared to use of a buffer without the water-soluble monomer or zwitterionic surfactant.