Dual-Mechanism Antibacterial Composition for Broad pH Efficacy
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Solution Overview
Problem
Existing antibacterial agents based on 2-phenylbenzimidazole compounds are ineffective in acidic and neutral environments where V-ATPase expression is low or absent, limiting their applicability.
Innovation Solution
Combining a 2-phenylbenzimidazole-based compound with a salicylanilide-based compound to create an antibacterial composition that inhibits Na+-transporting V-ATPase and F-ATPase activities across various pH environments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a 2-phenylbenzimidazole-based compound is used as an antibacterial agent, then it effectively inhibits V-ATPase in alkaline environments, but it becomes ineffective in acidic and neutral environments where V-ATPase is not expressed
Solution Approach 1:
The patent combines a 2-phenylbenzimidazole-based compound (V-ATPase inhibitor) with a salicylanilide-based compound (protonophore) to create a composite antibacterial composition. The 2-phenylbenzimidazole component targets V-ATPase in alkaline environments, while the salicylanilide component acts as a protonophore in acidic and neutral environments, allowing the combination to effectively cover all pH conditions where pathogenic bacteria may be present.
2Object-affected harmful factors
If the antibacterial agent targets V-ATPase specifically, then it achieves selective inhibition of pathogenic bacteria, but it fails to function in environments where V-ATPase expression is low or absent
Solution Approach 1:
The antibacterial composition achieves multi-functionality by incorporating two compounds with different mechanisms: the 2-phenylbenzimidazole-based compound specifically inhibits V-ATPase in alkaline environments, while the salicylanilide-based compound functions as a protonophore across all pH ranges. This dual-mechanism approach ensures the composition remains effective regardless of environmental pH conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The combined composition effectively inhibits bacterial proliferation in a wide pH range, including acidic and neutral environments, targeting drug-resistant bacteria and maintaining antibacterial efficacy.
Implementation Method 1
a 2-phenylbenzimidazole-based compound exhibits an activity inhibitory function of Na + -transporting V-ATPase
Implementation Method 2
a protonophore containing a salicylanilide-based compound
Implementation Method 3
the V-ATPase has a function to hydrolyze ATP and to transfer intracellular sodium ions
Implementation Method 4
transports ions between the inside and outside of the membrane while rotating a specific detail unit in the membrane by ATP hydrolysis energy
Data Source
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AI summary
The present invention provides an antibacterial composition containing a 2-phenylbenzimidazole-based compound and a salicylanilide-based compound. In addition, the present invention provides an antibacterial method for inhibiting proliferation of bacteria using the antibacterial composition, the method including: administering the 2-phenylbenzimidazole-based compound and the salicylanilide-based compound to bacteria having Na+-transporting V-ATPase and F-ATPase; and binding the 2-phenylbenzimidazole-based compound to the V-ATPase of the bacteria to inhibit activity of the V-ATPase, and breaking a proton concentration gradient by the F-ATPase with the salicylanilide-based compound to inhibit activity of the F-ATPase.