Dual-Conjugate Rapid Test Strip for Clear Result Differentiation
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Solution Overview
Problem
Conventional competitive lateral flow immunoassay rapid test reagents face challenges in accurately distinguishing between positive and negative results due to suboptimal antibody-antigen affinity, leading to potential false positives and false negatives.
Innovation Solution
A rapid test strip design featuring a base layer with sequential layers of a sample pad, a first conjugate pad with gold-conjugated antibody complexes, and a second conjugate pad with gold-conjugated trithiocyanuric acid complexes, enhancing detection signal intensity through sulfur-gold bonds.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Illumination intensity
If conventional competitive lateral flow immunoassay rapid test reagent is used, then the test can be performed with simple structure, but the detection signal intensity is insufficient leading to difficulty in distinguishing positive and negative results
Solution Approach 1:
The conjugate pad is divided into two separate pads: a first conjugate pad containing gold-conjugated antibody complexes and a second conjugate pad containing gold-conjugated trithiocyanuric acid complexes. This segmentation allows each pad to perform a specific function in the detection process, enhancing the overall detection signal intensity while maintaining a relatively simple structure.
Solution Approach 2:
The invention uses composite material systems including gold-conjugated antibody complexes and gold-conjugated trithiocyanuric acid complexes. These composite materials combine different functional components to create a synergistic effect that enhances detection signal intensity compared to using a single material system.
2Measurement precision
If antibody and antigen affinity is not optimized, then the test reagent can be manufactured with simpler processes, but the accuracy of result distinction is compromised
Solution Approach 1:
The invention changes the chemical parameters of the conjugate system by introducing trithiocyanuric acid complexes alongside antibody complexes. This parameter change in the conjugate composition enhances the detection signal and improves result distinction accuracy without significantly complicating the manufacturing process.
3Illumination intensity
If single conjugate pad is used, then the device structure is simple, but the detection signal is not intense enough for clear positive/negative distinction
Solution Approach 1:
The single conjugate pad is segmented into two separate conjugate pads with different compositions. The first conjugate pad contains gold-conjugated antibody complexes while the second contains gold-conjugated trithiocyanuric acid complexes. This segmentation enables each pad to contribute differently to the detection signal, achieving higher intensity without excessive complexity.
Solution Approach 2:
The invention adds a dimensional aspect by introducing a second conjugate pad alongside the first one. This dimensional expansion in the test strip structure allows for enhanced detection signal through the combined action of two conjugate systems, improving the positive/negative distinction capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The design provides a high-intensity detection signal, clearly differentiating positive and negative results, reducing false positives and negatives by ensuring distinct color development, with a detection threshold of 20 ng/ml.
Implementation Method 1
enhancing detection signal intensity through sulfur-gold bonds
Implementation Method 2
A specimen first flows from the sample pad toward the absorption pad through the capillary action of fibers
Data Source
AI summary
A rapid test strip is provided, wherein the rapid test strip includes a base layer, a sample pad, a first conjugate pad, a second conjugate pad, a reaction pad and an absorption pad, the sample pad, the first conjugate pad, the second conjugate pad, the reaction pad and the absorption pad are sequentially disposed on the base layer along a first direction, the first conjugate pad includes a plurality of gold-conjugated antibody complexes, and the second conjugate pad includes a plurality of gold-conjugated trithiocyanuric acid complexes.