Dual Fluorescent Sample Observation Area Specification
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Solution Overview
Problem
Existing sample observation methods lack sufficient accuracy in specifying the analysis area based on the position of the cell nucleus, leading to inadequate analysis of samples using fluorescent light intensity.
Innovation Solution
A sample observation device and method that utilize dual fluorescent substances and lights, where the first fluorescent substance is in the sample and the second in the solution, allowing for precise area specification and analysis by generating and processing images from both lights to accurately define the analysis area.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If the area in which there is the sample is estimated only on the basis of the position of the cell nucleus, then the specification process is simple, but the accuracy of specifying the analysis area is insufficient
Solution Approach 1:
The patent introduces a second fluorescent substance as an intermediary marker in the solution to define the boundary of the analysis area. This intermediary element mediates between the simple specification process and accurate area definition, allowing the system to achieve high precision without excessive complexity by using the second fluorescent substance as a reference framework
Solution Approach 2:
The patent changes the parameter basis for area specification from solely nuclear position coordinates to a dual-parameter system combining nuclear position with the spatial distribution of the second fluorescent substance. This parameter transformation enables more accurate analysis area definition by incorporating additional spatial information from the fluorescent solution distribution
2Measurement precision
If dual fluorescent substances and lights are used to generate and process images for precise area specification, then the analysis accuracy is improved, but the device complexity increases
Solution Approach 1:
The patent makes the imaging system universal by enabling it to capture and process multiple fluorescent signals (first and second fluorescent substances) through the same optical path and imaging device. This multi-functionality allows the system to achieve high analysis accuracy by utilizing both fluorescent markers without requiring separate specialized devices for each, thereby managing complexity through resource sharing
Solution Approach 2:
The patent merges the detection of two different fluorescent substances into a unified imaging and processing workflow. By combining the optical paths, imaging devices, and analysis procedures for both fluorescent markers into an integrated system, the patent achieves high measurement precision while controlling device complexity through consolidation rather than multiplication of components
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high-accuracy analysis of samples by clearly delineating the analysis area within the sample observation device, improving the precision of fluorescent light intensity analysis.
Implementation Method 1
a sample observation device for observing a sample using fluorescent light... an irradiation unit that irradiates the well with first excitation light for exciting the first fluorescent substance and second excitation light for exciting the second fluorescent substance; an image formation unit that forms first fluorescent light generated in the sample due to the first excitation light and second fluorescent light generated in the solution due to the second excitation light as images
Data Source
AI summary
A sample observation device includes a sample container that holds the sample stained with a first fluorescent substance and a solution including a second fluorescent substance, an irradiation unit that performs irradiation with first excitation light and second excitation light, a scanning unit that scans the sample container in one direction, an image formation unit that forms first fluorescent light from the sample and second fluorescent light from the solution, an imaging unit that outputs first image data based on the first fluorescent light and first image data based on the second fluorescent light, an image processing unit that generates a first fluorescent light image based on a plurality of pieces of first image data and a second fluorescent light image based on a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample on the basis of the second fluorescent light image and sets an analysis area in the first fluorescent light image.