Dual-Mode Analyte Detection Device for Simultaneous Semi-Quantification
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Solution Overview
Problem
Existing devices and methods for detecting analytes in samples are limited in their ability to simultaneously and semi-quantitatively detect multiple analytes using a single device.
Innovation Solution
A device comprising a first test portion with a test membrane for chemical assays and a second test portion with a conjugate pad and immunoreaction membrane for immunoassays, allowing for the detection of two analytes of interest in a single sample through vertical and lateral flow assays.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a single device is used to detect multiple analytes, then detection efficiency is improved, but device complexity increases
Solution Approach 1:
The patent combines a chemical assay test portion and an immunoassay test portion into a single device, allowing simultaneous detection of multiple analytes (e.g., glucose and hCG) in one sample application, thereby improving detection efficiency while integrating multiple functions into one unified device structure
Solution Approach 2:
The device is designed with multi-functional test portions that can detect different types of analytes using different assay methods (chemical and immunoassay) within a single device, enabling universal detection capabilities across multiple analyte types without requiring separate devices
2Measurement precision
If semi-quantitative detection is implemented, then measurement capability is improved, but device complexity increases
Solution Approach 1:
The patent employs color intensity changes in test signals that are directly proportional to analyte concentrations, with color indicators provided for comparison to enable semi-quantitative measurement. This allows measurement capability improvement through visual colorimetric analysis without requiring complex electronic sensors or instrumentation
Solution Approach 2:
The device enables self-service semi-quantitative measurement through built-in color indicators and test signals that automatically provide concentration information through color intensity comparison, eliminating the need for external measurement equipment or complex data processing systems
3Adaptability or versatility
If multiple test portions are integrated in one device, then detection versatility is improved, but manufacturing complexity increases
Solution Approach 1:
The device is divided into separate functional test portions (chemical assay portion and immunoassay portion) that can be independently designed and manufactured, then assembled into a complete device. This segmentation allows each portion to be optimized for its specific assay type while simplifying the overall manufacturing process through modular assembly
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables simultaneous detection and semi-quantification of multiple analytes in a single sample, improving efficiency and accuracy in analyte detection.
Implementation Method 1
causing the sample to flow vertically onto a test membrane
Implementation Method 2
causing the sample to flow laterally from the test membrane onto a conjugate pad
Implementation Method 3
The second analyte of interest, if present in the sample, may be caused to bind to the detection particles at the conjugate pad, thereby forming a complex
Implementation Method 4
The complex if formed may be caused to bind to the one or more test antibodies to produce a test signal at the test zone
Implementation Method 5
The sample may be caused to chemically react with a reaction mixture blotted on the test membrane to produce a test signal at a chemical test signal zone
Data Source
AI summary
A device and method for detecting a plurality of analytes of interest in a sample are disclosed. The device has a first portion for detection of a first analyte of interest, and a second test portion for detection of a second analyte of interest. The first test portion has a test membrane with a chemical reaction side having a reaction mixture blotted thereon. The second test portion has a conjugate pad having detector particles bound thereon and an immunoreaction membrane having a test zone comprising a test antibody bound thereon, and a control zone comprising a control antibody bound thereon. The first test portion is in fluid-flow contact with the second test portion, arranged for transporting the sample away from the first test portion towards the second test portion.