Dual-Mode Electrochemical Biosensor for Point-of-Care Pathogen Profiling
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Solution Overview
Problem
Current laboratory-based diagnostics for bacterial infections are slow, require specialized equipment, and yield inaccurate results, exacerbating the antibiotic resistance crisis due to misdiagnosis and misuse of antibiotics.
Innovation Solution
A dual-mode electrochemical biosensor device integrating a pathogen identification and quantification unit with a profiling unit, using monoclonal antibodies and substrate-based biosensors to detect and characterize pathogenic biomarkers and enzymatic activity, enabling rapid, cost-effective, and sensitive point-of-care diagnostics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If laboratory-based diagnostics are used for bacterial infection detection, then detection capability is provided, but processing time is long and equipment complexity is high
Solution Approach 1:
The patent replaces complex mechanical laboratory equipment with an electrochemical biosensor system that uses electrical signals for pathogen detection. The biosensor employs electrochemical impedance spectroscopy to detect bacterial cells, eliminating the need for complex mechanical instrumentation while achieving rapid results within minutes.
Solution Approach 2:
The patent changes the detection parameter from traditional optical or mechanical measurements to electrochemical impedance measurements. By measuring changes in electrical impedance caused by bacterial cell binding to functionalized electrodes, the system achieves rapid detection without requiring lengthy processing times associated with traditional laboratory methods.
2Measurement precision
If laboratory-based diagnostics are used for bacterial infection detection, then detection capability is provided, but equipment specialization and cost are high
Solution Approach 1:
The patent substitutes complex specialized laboratory equipment with a portable electrochemical biosensor that uses simple electrical measurements. The system replaces sophisticated mechanical and optical instrumentation with an electrochemical platform that can be performed in point-of-care settings without requiring specialized facilities.
Solution Approach 2:
The patent designs a universal electrochemical biosensor platform that can detect multiple pathogens and biomarkers using the same fundamental electrochemical detection principle. This multi-functional system eliminates the need for multiple specialized equipment by integrating various detection capabilities into a single portable platform.
3Productivity
If traditional diagnostic methods are used, then pathogen detection is attempted, but accuracy is low leading to misdiagnosis
Solution Approach 1:
The patent applies local quality by functionalizing the electrode surface with specific antibodies or receptors that are highly selective for particular pathogen antigens. This localized molecular recognition at the electrode interface enables accurate differentiation between pathogenic and non-pathogenic bacteria, significantly improving diagnostic accuracy while maintaining high throughput.
Solution Approach 2:
The patent incorporates feedback mechanisms through electrochemical impedance spectroscopy that provides real-time information about bacterial cell binding events. The system continuously monitors impedance changes and uses this feedback to confirm positive identifications and quantify pathogen load, ensuring both accuracy and productivity in diagnostic results.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device provides rapid, accurate identification and quantification of antibiotic-resistant pathogens, simplifying sample preparation and allowing for straightforward measurement of complex matrices, thereby supporting effective treatment regimens.
Implementation Method 1
The present disclosure describes a selected application of a dual-mode electrochemical platform that contains two electrochemical biochips... performing electrochemical impedance spectroscopy (EIS), enabling to provide data on presence and/or quantity of captured pathogen
Implementation Method 2
The interaction of the at least one substrate molecule/s with at least one catalytic macromolecule, catalyzes the production of at least one electroactive product
Data Source
AI summary
The present invention relates to diagnostic platform. More specifically, the invention relates to a dual platform composed of a detection and quantification unit and a characterization unit, systems, kits, methods and uses thereof in detection, quantification and characterization of pathogens, the system comprising monoclonal-antibody-based biosensor chips, for detection and/or quantification of pathogens in a sample, and substrate-based biosensor chips for enzymatically profiling the pathogen in the sample.