Dual-Sensor Imaging Layout for High-Resolution Fluorescence Observation
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Solution Overview
Problem
Existing imaging technologies face challenges in capturing visible light with high resolution while clearly capturing fluorescence, as increasing pixel density for high-resolution imaging reduces light reception per pixel, and fluorescence is often too weak to be captured effectively.
Innovation Solution
An imaging device with a prism that separates visible light and fluorescence, using a visible-light imaging sensor and a fluorescence imaging sensor with specific pixel spacing ratios to ensure high-resolution visible light capture and clear fluorescence capture, aided by a long-pass filter to block unnecessary visible light.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the number of pixels of an imaging sensor is increased for high-resolution imaging, then imaging resolution is improved, but the light receiving area per pixel is narrowed and sensitivity becomes insufficient
Solution Approach 1:
The imaging device is divided into two separate imaging sensors: a visible-light imaging sensor optimized for high-resolution visible light capture, and a fluorescence imaging sensor optimized for fluorescence capture with larger pixel spacing. This segmentation allows each sensor to be independently optimized for its specific function, resolving the contradiction between resolution and sensitivity.
2Illumination intensity
If pixel spacing in fluorescence imaging sensor is increased to improve sensitivity, then fluorescence capture capability is improved, but imaging resolution deteriorates
Solution Approach 1:
Different regions of the imaging system are assigned different quality characteristics: the visible-light imaging sensor uses small pixel spacing for high resolution, while the fluorescence imaging sensor uses large pixel spacing for high sensitivity. Each sensor's pixel spacing is locally optimized for its specific imaging task, allowing both high resolution and high sensitivity to coexist in different parts of the system.
3Device complexity
If a single imaging sensor is used for both visible light and fluorescence, then device complexity is reduced, but the ability to clearly capture both types of light simultaneously deteriorates
Solution Approach 1:
The imaging system is segmented into two dedicated sensors rather than using a single multi-purpose sensor. This segmentation, combined with a dichroic mirror that separates visible light and fluorescence paths, allows each sensor to be optimized for its specific wavelength range, achieving superior dual-mode imaging quality despite increased device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves high-resolution visible light capture and clear fluorescence capture by optimizing pixel spacing ratios and using a long-pass filter, enhancing sensitivity and image quality.
Implementation Method 1
a prism that separates visible light and fluorescence included in light from a target to be observed
Implementation Method 2
aided by a long-pass filter to block unnecessary visible light
Data Source
AI summary
An imaging device includes a prism that separates visible light and fluorescence included in light from a target to be observed, a visible-light imaging sensor that captures the visible light separated by the prism, and a fluorescence imaging sensor that captures the fluorescence separated by the prism, in which a ratio of a pixel spacing in the fluorescence imaging sensor to a pixel spacing in the visible-light imaging sensor is 1 or more and less than 5.