Plant Stress Diagnosis With Dual-Wavelength ROS Detection

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Conventional chlorophyll fluorescence measurement primarily detects photosystem II activity, failing to accurately assess downstream electron transfer processes, particularly reactive oxygen species (ROS) production, which limits effective environmental stress diagnosis in plants.

Innovation Solution

An environmental stress diagnosis device utilizing dual-wavelength light absorption difference measurement and synchronized opposite-phase rectangular waves to measure ROS markers, combined with oxygen production rate analysis, for accurate and early environmental stress diagnosis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If chlorophyll fluorescence measurement is used to detect photosystem II activity, then photosynthetic activity can be measured, but reactive oxygen species production cannot be accurately detected

Engineering Contradiction:

Improvephotosynthetic activity detectionVSAvoiddownstream electron transfer information

Core Design Contradiction:

Measurement precisionVSLoss of information

Solution Approach 1:

The patent divides the measurement into two distinct parts: chlorophyll fluorescence measurement for photosystem II activity and light absorption difference measurement for photosystem I and ROS detection. This segmentation allows each measurement method to target specific physiological processes, thereby capturing complete photosynthetic information including downstream electron transfer and ROS production that were previously missed

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent creates a multi-functional diagnosis system that integrates multiple measurement capabilities (photosystem II detection, photosystem I detection, and ROS detection) into a single comprehensive environmental stress diagnosis device. This universal approach enables the device to perform multiple diagnostic functions simultaneously, providing holistic plant health assessment

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Loss of time

If conventional chlorophyll fluorescence measurement is used, then early health condition finding is enabled, but accurate environmental stress diagnosis is limited

Engineering Contradiction:

Improvediagnosis timingVSAvoidenvironmental stress diagnosis accuracy

Core Design Contradiction:

Loss of timeVSMeasurement precision

Solution Approach 1:

The patent performs preliminary measurements of both chlorophyll fluorescence and light absorption differences under controlled conditions to establish baseline data and diagnostic criteria. By preparing these reference measurements in advance, the system can quickly compare subsequent measurements against established patterns, enabling both early detection and accurate diagnosis of environmental stress conditions

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables precise and timely detection of environmental stress and mineral nutrient deficiencies in plants, facilitating stress-tolerant variety selection and cost-effective cultivation management.

Implementation Method 1

a measurement light source that radiates a measurement light to a plant sample; an induction light source that radiates a photosynthesis inducing light to the plant sample; a transmitted light detector that detects the measurement light transmitted through the plant sample as a transmitted light

Methodology Applied

Scientific EffectLight absorption: Absorption (EM radiation)

Implementation Method 2

how many electrons are produced from water molecules in a photochemical reaction can be quantitatively identified by monitoring slight light energy (chlorophyll fluorescence) emitted from chlorophyll

Methodology Applied

Scientific EffectPhotosynthesis: Photosynthesis

Implementation Method 3

monitoring slight light energy (chlorophyll fluorescence) emitted from chlorophyll

Methodology Applied

Scientific EffectChlorophyll fluorescence: Fluorescence

Data Source

PatentUS12405258B2Diagnosis device for environmental stress in plants and environmental stress diagnosis method

Publication Date: 2025.09.02 BUNKOU KEIKI

AI summary

The present invention relates to an improved technology for a device that identifies and diagnoses an environmental stress state of plants. A control circuit 20b controls a measurement light source 12 such that a second measurement light ML2 has higher power than a first measurement light ML1 and the first measurement light ML1 and the second measurement light ML2 become opposite-phase rectangular waves, and further controls the measurement light source 12 such that the first measurement light ML1 and the second measurement light ML2 are output in synchronization to form the first measurement light ML1 and the second measurement light ML2 into a quasi-single composite rectangular wave measurement light ML3 of 5 kHz to 30 kHz. A transmitted light detector 18 detects the composite rectangular wave measurement light ML3 transmitted through a plant sample S as a composite rectangular wave transmitted light TL. An analysis circuit 20a calculates a light absorption difference by utilizing the composite rectangular wave transmitted light TL, and calculates Y(ND) which is an oxidized state of P700 as a ROS marker utilizing the light absorption difference, and diagnoses an environmental stress state of plants by utilizing the ROS marker.