DUF2996 Protein Expression for Consistent Crop Yield
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional methods for improving crop yield, such as selective breeding, are labor-intensive and often result in heterogeneous genetic components that do not consistently pass desirable traits to offspring, while genetic engineering can be precise but may also impair plant growth and development.
Innovation Solution
Increasing the expression of a gene encoding a DUF2996 domain-containing protein in plants through genetic transformation, using specific coding sequences and promoters to enhance crop yield by modulating metabolic pathways involved in plant growth and biomass accumulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If selective breeding techniques are used to improve crop yield, then desirable traits can be identified in plants, but the process is labor intensive and results in heterogeneous genetic components that do not consistently pass traits to offspring
Solution Approach 1:
The patent replaces conventional mechanical selective breeding methods with genetic engineering technology. Specifically, it introduces and expresses a DUF2996 domain-containing protein through genetic transformation, directly modifying plant germplasm to achieve desired traits (increased biomass and yield) without relying on labor-intensive cross-breeding and selection processes
Solution Approach 2:
The patent changes the genetic parameters of the plant by introducing a specific gene encoding the DUF2996 domain-containing protein. This genetic modification alters the plant's metabolic pathways and developmental processes, resulting in consistent expression of desirable traits (increased yield and biomass) that are reliably passed to offspring through stable genetic integration
2Manufacturing precision
If genetic engineering is used to precisely modify plant germplasm, then crops with improved traits can be developed, but plant growth and development may be impaired
Solution Approach 1:
The patent uses the DUF2996 domain-containing protein as an intermediary molecule to mediate the genetic engineering process. This protein, when expressed in the plant, acts as a functional mediator that enhances metabolic pathways and developmental processes without causing harmful side effects, thus maintaining plant growth stability while achieving precise genetic modification goals
Solution Approach 2:
The introduced DUF2996 domain-containing protein performs self-regulating functions within the plant system. It autonomously participates in metabolic pathways and developmental processes, enhancing plant growth and yield without requiring external intervention or causing disruption to the plant's natural growth rhythms and developmental stability
3Productivity
If gene expression is modulated to increase plant biomass and yield, then crop production can be improved, but growth and development may be impaired relative to control plants
Solution Approach 1:
The patent applies partial overexpression of the DUF2996 domain-containing protein through controlled gene expression systems. By using promoters that drive moderate to high levels of expression (without complete overexpression), the patent achieves enhanced biomass and yield while avoiding the harmful effects of excessive protein accumulation, thus maintaining growth consistency
Solution Approach 2:
The patent implements spatially and temporally controlled expression of the DUF2996 domain-containing protein using tissue-specific or developmentally-regulated promoters. This localized expression strategy ensures that the protein is produced in specific plant tissues or at specific developmental stages where it can enhance yield without interfering with overall plant growth and development consistency
Data Source
AI summary
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding DUF2996 domain-containing protein s, polypeptides encompassing DUF2996 domain-containing protein s, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.