Duodenal Juice Sampling for Non-Invasive Disease Detection
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Solution Overview
Problem
Conventional methods for detecting early stages of upper gastrointestinal diseases like pancreatic cancer and biliary tract cancer are invasive, costly, and have low detection accuracy due to the need for pancreatic juice and bile collection, which requires skilled procedures and can cause pancreatitis, and the use of stimulants leading to prolonged examination times and protease inhibitor inefficacy.
Innovation Solution
A method involving the collection of duodenal juice using sampling equipment inserted into the duodenum, where the juice is naturally secreted and collected without stimulants, with a predetermined quantity of 0.001 ml to 3.0 ml, and analyzed using immunochromatography for protein detection within five minutes to prevent protease activation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If pancreatic juice or bile is collected using catheter insertion into ducts, then detection accuracy for upper gastrointestinal diseases is improved, but the procedure becomes invasive and may cause pancreatitis
Solution Approach 1:
The patent extracts the essential function of obtaining pancreatic juice and bile while eliminating the harmful catheter insertion step. Instead of directly accessing the ducts, the method collects duodenal juice which naturally contains pancreatic juice and bile, achieving the same detection purpose without the invasive procedure and pancreatitis risk
Solution Approach 2:
The patent introduces duodenal juice as an intermediary medium. Rather than directly collecting pancreatic juice or bile through invasive catheterization, the method uses duodenal juice (which naturally contains pancreatic juice and bile) as a non-invasive intermediary to achieve the same detection objectives
2Measurement precision
If stimulant administration is used to enhance pancreatic juice secretion, then detection sensitivity is improved, but examination time is prolonged and patient burden increases
Solution Approach 1:
The patent removes the stimulant administration step from the examination protocol. By collecting duodenal juice during the natural secretion phase without requiring secretin or other stimulants, the method achieves adequate detection sensitivity while significantly reducing examination time and patient burden
Solution Approach 2:
The patent applies partial action by collecting duodenal juice for a limited duration (5-10 minutes) during natural secretion rather than requiring prolonged stimulation. This partial collection period is sufficient to obtain adequate sample volume for detection without the need for extended stimulant administration
3Stability of the object's composition
If protease inhibitor is added to duodenal juice after collection, then protein preservation is improved, but proteins and cells may be broken down during the collection and accumulation period
Solution Approach 1:
The patent applies preliminary action by adding protease inhibitor to the duodenal juice collection container at the time of collection rather than after. This preliminary addition immediately prevents protease activation and protein degradation during the collection and accumulation process, ensuring protein integrity for accurate detection
Solution Approach 2:
The patent applies preliminary anti-action by pre-addition of protease inhibitor to counteract the harmful protease activity in the duodenal juice. This preliminary protective measure prevents protein and cell breakdown during collection, maintaining sample quality for accurate detection
4Measurement precision
If catheter insertion is performed for pancreatic juice collection, then detection capability is improved, but the procedure requires significant doctor skill and is not easily performable
Solution Approach 1:
The patent extracts the detection capability from the complex catheter insertion procedure. By using duodenal juice collection through standard endoscopic techniques rather than requiring skilled catheterization, the method maintains detection capability while significantly simplifying the procedure and reducing the skill level required
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method simplifies the examination process, reduces patient and doctor burden, improves detection accuracy, and allows for efficient collection and analysis of duodenal juice for early disease detection without causing harm, providing a non-invasive and cost-effective solution for upper digestive system diseases.
Implementation Method 1
collecting duodenal juice naturally secreted in the duodenum using the sampling equipment
Implementation Method 2
detecting concentration of a protein included in the collected duodenal juice using an immunochromatography method
Implementation Method 3
if the protease inhibitor is added to the collected duodenal juice after the duodenal juice has been collected for 10 minutes or 15 minutes at a time over a one-hour period, the proteins and cells to be measured are broken down by the protease activated in the duodenal juice
Data Source
AI summary
A method of collecting a specimen of the present invention is used in detecting upper digestive system disease. The method of collecting a specimen includes: a step of positioning sampling equipment in the duodenum of the subject into which duodenal juice is secreted, the sampling equipment being used to collect and store the duodenal juice; a step of collecting duodenal juice naturally secreted in the duodenum using the sampling equipment; and a step of stopping collection of the duodenal juice when collection quantity of the duodenal juice reaches a predetermined quantity which is 3.0 ml or less.