Dyella-like Bacterium Composition for Phytoplasma Control

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Solution Overview

Problem

Conventional methods for controlling phloem-restricted pathogens like phytoplasma in grapevines are inefficient, and there is a need for new strategies to manage diseases that affect wine quality and grape production.

Innovation Solution

An isolated bacterium with specific nucleic acid sequences, referred to as the Dyella-like bacterium, is used in compositions that include emulsifiers and growth substrates, which exhibit anti-pathogenic activity against phytopathogens, including phytoplasma, by reducing symptoms and penetrating plant tissues through roots, seeds, and leaves.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional pesticides are used to control phytoplasma, then disease control is attempted, but the control is inefficient and wine quality consistency deteriorates

Engineering Contradiction:
Improvedisease control effectivenessVSAvoidwine quality consistency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent introduces a bacterial endophyte as an intermediary organism that colonizes plant tissues and produces antimicrobial compounds. This endophyte acts as a mediator between the plant and phytoplasma pathogen, providing protection through natural biological mechanisms rather than direct chemical intervention, thereby maintaining wine quality while controlling disease

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces the mechanical/chemical system of conventional pesticide application with a biological system using endophytic bacteria. The biological control mechanism involves the endophyte colonizing plant tissues and producing antimicrobial substances naturally, substituting the need for external chemical pesticides and their associated quality impacts

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If new biological control strategies are developed, then disease control effectiveness improves, but the complexity of the control system increases

Engineering Contradiction:
Improvedisease control effectivenessVSAvoidcontrol system complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent employs self-service principles where the endophytic bacteria autonomously colonize plant tissues and produce antimicrobial compounds without requiring complex external management. The biological control system serves itself by utilizing the endophyte's natural abilities to establish protection, reducing the need for complex application and monitoring systems

Inventive Principle:
Principle #25Self-service

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The bacterium composition effectively inhibits the growth of phytopathogens, such as phytoplasma, and reduces disease symptoms in grapevines, demonstrating potential as a biological control agent for improving grapevine health and wine quality.

Implementation Method 1

penetrating plant tissues through roots, seeds, and leaves

Methodology Applied
Scientific EffectBiological penetration:

Implementation Method 2

inhibits the growth of phytopathogens, such as phytoplasma

Methodology Applied
Scientific EffectAntimicrobial inhibition:

Data Source

PatentUS20240240138A1Anti-phytopathogenic compositions
Publication Date: 2024.07.18 THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT
  • US20240240138A1 patent drawing
  • US20240240138A1 patent drawing
  • US20240240138A1 patent drawing

AI summary

The present invention relates to an isolated bacterium and composition comprising same having anti-pathogenic activity. The invention further relates to compounds derived from said bacterium or analogs thereof, and methods of using same for treating or reducing the symptoms of a pathogen including but not limited to a phytopathogen.