Dynamic Analyte Binding Measurement for Faster Selective Sensing
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Solution Overview
Problem
Existing biological and chemical assays face challenges in improving selectivity and sensitivity, particularly for biological sensing systems, with slow binding processes and difficulty in differentiating between biomolecules with minor changes, and require long incubation times for equilibrium, limiting point-of-care applications.
Innovation Solution
A method and system that applies a field to cause analytes to debind from capture species on a sensing surface, allowing for dynamic measurement signals during binding and debinding steps, enabling faster and more accurate determination of analyte properties by applying forces to manipulate binding and debinding interactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional sensors use immobilized capture species with covalent or non-covalent interactions, then selectivity and sensitivity are improved, but incubation time increases to 30 minutes or more
Solution Approach 1:
The patent applies dynamic field application (electrical, magnetic, or acoustic) to actively manipulate the binding and debinding processes. By dynamically switching fields on and off, the system accelerates both association and dissociation rates, reducing incubation time from 30+ minutes to significantly shorter durations while maintaining measurement precision through controlled dynamic interactions between capture species and analytes.
Solution Approach 2:
The patent changes physical parameters by applying external fields (electrical, magnetic, or acoustic) to modify the binding characteristics of capture species. These field applications alter the energy states and interaction forces, enabling faster equilibrium reaching without sacrificing selectivity and sensitivity, thus resolving the time-precision tradeoff.
2Measurement precision
If traditional assays require binding equilibrium to be reached, then accurate measurement is achieved, but time consumption increases significantly
Solution Approach 1:
The patent employs periodic application and removal of external fields to drive repeated binding and debinding cycles. This periodic action allows the system to reach measurement equilibrium faster by continuously refreshing the binding interfaces, achieving accurate measurements in reduced time compared to static equilibrium approaches.
Solution Approach 2:
The patent maintains continuous useful action through uninterrupted field application during the measurement process. By continuously applying fields to promote binding and then removing them to enable debinding, the system eliminates idle waiting periods and achieves rapid equilibrium while maintaining measurement accuracy throughout the process.
3Stability of the object's composition
If capture species are immobilized through strong chemical interactions, then binding stability is improved, but debinding difficulty increases
Solution Approach 1:
The patent replaces purely chemical binding mechanisms with a hybrid system that uses external physical fields (electrical, magnetic, or acoustic) to control debinding. This substitution allows strong chemical bonds to be formed for stable binding, while external fields provide a non-invasive means to induce debinding when needed, resolving the contradiction between binding stability and debinding ease.
Solution Approach 2:
The patent applies preliminary anti-action by using external fields to pre-prepare the system for debinding. Before actual debinding is needed, fields are applied to weaken or reversibly modify the binding interactions, making subsequent debinding easier while maintaining strong binding stability during the measurement phase.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances measurement accuracy and speed by providing information on binding affinities and concentrations through dynamic tracking of binding and debinding, reducing incubation times, and distinguishing between specific and non-specific bindings.
Implementation Method 1
applying a field to the sensing surface in a debinding step, the field being configured to apply a force to cause at least a portion of the specifically-bound analyte species to debind from the capture species
Implementation Method 2
capture species configured to specifically bind with the analyte provided on the sensing surface
Implementation Method 3
These species are used to capture target analytes, with the binding of analytes causing a change in a measured output
Data Source
AI summary
Methods and systems for determining a property of an analyte in a sample, wherein a field is applied to a sensing surface to cause an analyte to debind from a surface, and subsequently the field is modified or removed to allow the analyte to rebind.