Dynamic Charging Timing for Flow Cytometer Droplet Sorting

The patent applies the dynamics principle by transitioning from a fixed time delay system to a dynamic variable time delay system. The time delay is now calculated individually for each particle based on its measured velocity and trajectory, allowing the charging timing to adapt to each particle's specific path through the fluid stream. This resolves the contradiction by maintaining operational simplicity through automated calculation while achieving high sorting accuracy through particle-specific timing adjustment.

The patent implements parameter changes by varying the time delay parameter based on measured particle characteristics. Instead of using a constant time delay, the system calculates a specific delay for each particle using its velocity, position, and geometric parameters of the fluid stream. This allows the system to maintain simple operation while achieving accurate sorting by adjusting the timing parameter to match each particle's actual trajectory.

The patent applies feedback by using the measured particle velocity and position information to adjust the charging timing. The system senses particle characteristics, calculates the appropriate time delay based on these measurements and geometric parameters, and then applies the charge at the optimally timed moment. This feedback loop maintains relatively simple device architecture while significantly improving sample purity through accurate, particle-specific charging timing.

The patent implements preliminary action by calculating the time delay before the actual charging event occurs. The system measures particle properties, computes the required delay using geometric parameters and velocity data, and then executes the charging at the precisely calculated moment. This preliminary calculation step enables accurate sorting without requiring complex real-time adjustment mechanisms during the charging process itself.

The patent applies dynamics by making the time delay variable rather than fixed, allowing each particle to receive charging at its optimal moment based on its specific trajectory. This dynamic adjustment improves sorting efficiency and reduces dilution by ensuring that each droplet is charged at the precise moment when the target particle is in the correct position, thereby maintaining high processing speed while enhancing overall productivity.

The patent implements parameter changes by adjusting the time delay parameter for each particle based on its velocity and position measurements. This allows the system to process particles efficiently without the dilution problems associated with fixed timing, as each particle's charging time is optimized individually. The result is improved productivity through reduced sample dilution while maintaining high processing speeds.