Dynamic Filtration for High-Yield Protein Extraction
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Solution Overview
Problem
Current methods for extracting proteins like immunoglobulins from plasma-derived precipitates face challenges such as low yield, high impurity levels, and inefficiencies in purification, particularly due to issues with membrane filtration systems that foul easily and have suboptimal recovery rates.
Innovation Solution
A method involving a dynamic filtration system with a rotational cross-flow filter element and ceramic membranes, where the precipitate is mixed with a liquid to form a suspension, and then filtered to produce a permeate enriched with the protein of interest, with continuous dilution and recirculation to enhance extraction efficiency and minimize impurities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If membrane filtration systems are used for protein extraction, then purification can be achieved, but the membranes foul easily and recovery rates are suboptimal
Solution Approach 1:
The invention extracts and removes the problematic membrane filtration step from the protein extraction process. Instead of using membranes that foul and have suboptimal recovery, the process directly filters the suspension through a system that achieves both high purification and high recovery rates without membrane fouling issues.
Solution Approach 2:
The invention changes the physical and chemical parameters of the extraction process by using specific pH conditions (pH 3.0-5.0), temperature control (0-25°C), and solvent composition (acetone, ethanol, isopropanol) to optimize protein recovery while maintaining purification, replacing the membrane filtration approach.
2Reliability
If ethanol fractionation is used to separate plasma proteins, then purification can be achieved, but impurity levels remain high and yield is reduced
Solution Approach 1:
The invention optimizes the fractionation parameters by controlling pH (3.0-5.0), temperature (0-25°C), and solvent type/Concentration to achieve both high purification quality and high protein yield, improving upon traditional ethanol fractionation methods that sacrifice one for the other.
Solution Approach 2:
The invention uses dynamic filtration with adjustable parameters including flow rate control, temperature variation, and pH adjustment during the extraction process to simultaneously maximize both purification quality and protein yield, rather than using static fractionation conditions.
3Productivity
If dynamic filtration with rotational cross-flow filter element is used, then extraction efficiency is enhanced, but system complexity increases
Solution Approach 1:
The invention employs a rotational cross-flow filter element that creates dynamic filtration conditions, enhancing extraction efficiency by preventing fouling and improving mass transfer, while the rotational mechanism itself is a relatively simple mechanical addition to the filtration system.
Solution Approach 2:
The invention uses fluid dynamics and pressure control in the rotational cross-flow filtration system to enhance extraction efficiency, utilizing hydraulic principles to maintain optimal flow conditions without requiring complex mechanical or electronic control systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach achieves high recovery rates of proteins like immunoglobulin G, with up to 98% yield, while maintaining purity and reducing operational costs and footprint, by continuously shifting the solubility equilibrium and using a closed system for efficient protein extraction and purification.
Implementation Method 1
a first filtration unit comprising a dynamic filter element adapted to produce a first retentate and a first permeate enriched with the protein of interest
Implementation Method 2
A method involving a dynamic filtration system with a rotational cross-flow filter element
Implementation Method 3
by continuously shifting the solubility equilibrium and using a closed system for efficient protein extraction and purification
Data Source
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AI summary
The present invention relates to a method and a system of extracting a protein with high yield from a protein-comprising precipitate, in particular immunoglobulin, from human or non-human origins, such as blood plasma.