Dynamic Stimulation Control for Cell Observation Accuracy
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Solution Overview
Problem
Current observation apparatuses are inadequate in dynamically adjusting stimulation positions based on the position and shape of cells, leading to suboptimal observation and stimulation accuracy.
Innovation Solution
An observation apparatus and method that utilize a light source, illumination optical system, wavelength selecting section, observation optical system, and control section to selectively excite, stimulate, and image fluorescent substances within cells, allowing for precise control of illumination wavelengths and areas to adapt stimulation positions according to cell changes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional observation apparatuses are used to observe cells, then basic imaging is achieved, but the stimulation position cannot be dynamically adjusted according to cell position and shape changes
Solution Approach 1:
The system continuously acquires cell images, extracts position and shape information, and uses this feedback to dynamically adjust the stimulation position. The control section updates the stimulation position based on extracted cell information, ensuring accurate targeting despite cell movement or shape changes.
Solution Approach 2:
The stimulation position is made dynamic rather than fixed. The system continuously updates the stimulation position coordinates based on real-time cell position and shape extraction, allowing the stimulation to adapt to changing cell locations and morphologies during observation.
2Adaptability or versatility
If multiple wavelengths are used for different processing steps, then functional versatility is improved, but system complexity increases
Solution Approach 1:
A single illumination optical system is designed to handle multiple wavelengths for different functions: excitation wavelength for fluorescent substance excitation, stimulation wavelength for cell stimulation, and observation wavelength for imaging. This multi-functional approach avoids the need for separate optical systems for each function.
Solution Approach 2:
The wavelength selecting section acts as an intermediary component that manages multiple wavelengths within a unified optical system. It selectively transmits or blocks specific wavelengths to the specimen, enabling versatile wavelength-based processing without requiring multiple independent illumination paths.
3Measurement precision
If real-time cell imaging and stimulation are performed, then observation accuracy is improved, but processing time increases
Solution Approach 1:
The system performs periodic cycles of image acquisition, cell position/shape extraction, and stimulation. By structuring the process as repeated discrete cycles rather than continuous operation, the system can efficiently manage processing steps while maintaining real-time observation capability.
Solution Approach 2:
The cell position and shape are extracted from images in advance before stimulation is applied. This preliminary extraction allows the stimulation parameters to be pre-calculated and prepared, reducing the time required during the actual stimulation phase and improving overall processing efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate detection, stimulation, and imaging of cells, improving observation accuracy by allowing real-time adjustment of stimulation positions and shapes, thereby enhancing the precision of cellular observation post-stimulation.
Implementation Method 1
light having a first wavelength used to excite the fluorescent substance that specifically binds to or is expressed in a stimulus target
Implementation Method 2
wavelength selecting section that selects the wavelength of the illumination light to be radiated onto the specimen
Data Source
AI summary
An observation apparatus and an observation method are provided. The observation apparatus includes a light source that emits illumination light used to observe a specimen to which a fluorescent substance that specifically binds to or is expressed in a stimulus target has been supplied, an illumination optical system that radiates the illumination light emitted from the light source 11 onto the specimen, a deflecting device that changes an area of the specimen to be irradiated with the illumination light, a wavelength selecting section that selects the wavelength of illumination light to be radiated onto the specimen, an observation optical system that collects light from the specimen, a detector that detects the light collected by the observation optical system, an image processing section that generates an image from the light detected by the detector, and a control section that controls these components.