Dynamic Stirring Apparatus for Uniform Cell Density Control
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Solution Overview
Problem
During cell culture, density variations occur due to differences in adhesive strength between cells and the culture vessel, as well as between cells, leading to inefficient cell proliferation.
Innovation Solution
A stirring method and apparatus that measure the ease of peeling between cells and the culture vessel, and between cells, to determine the optimal stirring process, which includes the presence, acceleration, and frequency of stirring to maintain uniform cell density.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If cells are seeded in a culture vessel without stirring, then cell adhesion to the vessel surface is maintained, but density variations occur due to differences in adhesive strength between cells and the vessel surface and between cells
Solution Approach 1:
The patent applies dynamic stirring to the culture vessel, transforming the static cell adhesion system into a dynamic one. By applying controlled acceleration to the culture vessel during cell culture, the system prevents density variations while maintaining efficient cell proliferation. The stirring unit moves the culture vessel in a stirring direction, creating dynamic conditions that prevent cell aggregation and ensure uniform cell distribution throughout the culture medium.
Solution Approach 2:
The patent changes physical parameters of the culture system by applying controlled acceleration to the culture vessel. The stirring process content determining unit calculates optimal acceleration parameters based on the ratio of adhesive strength between cells and the vessel surface to adhesive strength between cells. By adjusting these acceleration parameters, the system achieves uniform cell density while maintaining high cell proliferation efficiency.
2Stability of the object's composition
If stirring is applied to cells in the culture vessel, then uniform cell density is achieved, but the adhesive strength between cells and the vessel surface must be carefully controlled
Solution Approach 1:
The patent implements a feedback control system where the stirring process content determining unit continuously monitors the adhesive strength ratio between cells and the vessel surface and between cells. Based on this feedback, the system automatically calculates and adjusts the optimal stirring acceleration parameters. The control unit then executes the calculated stirring process, creating a closed-loop feedback system that maintains uniform cell density while simplifying operational control.
Solution Approach 2:
The system performs self-adjustment by automatically calculating optimal stirring parameters based on measured adhesive strength characteristics. The stirring process content determining unit uses the adhesive strength ratio to autonomously determine the appropriate acceleration and stirring duration, eliminating the need for manual intervention and reducing operational complexity while ensuring optimal cell density uniformity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method and apparatus effectively prevent density variations, enabling efficient cell culture by ensuring uniform cell distribution and proliferation.
Implementation Method 1
measuring the ease of peeling between the culture vessel and the cell by transporting the culture vessel at a predetermined acceleration and stopping the culture vessel
Data Source
AI summary
A stirring apparatus including a stirring mechanism configured to execute a stirring process on cells in a culture vessel, and a control unit programmed to at least one of control the stirring mechanism, determine the stirring process of the stirring mechanism, and determine whether the stirring process is performed or not.