Edible Bird's Nest Extract via Binding Moiety Purification
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Solution Overview
Problem
Current methods for processing edible bird's nest (EBN) result in low bioavailability of beneficial components due to large biomacromolecules being difficult to digest, potential allergenicity, contamination risks, and adulteration issues, which compromise quality and safety.
Innovation Solution
A method involving the use of specific binding moieties such as opsonin, complement protein, lectin, ficolin, and pentraxin binding moieties to extract and isolate bioactive molecules from EBN, enhancing bioavailability and purity while minimizing harmful effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If EBN is consumed in the form of soup or drinks by boiling in water, then the preparation is simple and traditional, but the bioavailability of beneficial components is low due to large biomacromolecules being difficult to digest and absorb
Solution Approach 1:
The patent applies segmentation by breaking down the large biomacromolecules of EBN into smaller peptide fragments through enzymatic hydrolysis. The complex protein structures are divided into smaller, more digestible units that can be easily absorbed by the body, thereby improving bioavailability while maintaining the traditional preparation approach.
Solution Approach 2:
The patent changes the molecular size parameter of EBN components by using enzymatic treatment to convert large proteins into smaller peptides. This parameter change from macromolecules to smaller fragments directly addresses the bioavailability issue while keeping the preparation method simple and suitable for traditional consumption forms.
2Adaptability or versatility
If whole EBN is consumed, then the traditional method is maintained, but it may lead to IgE mediated anaphylaxis and other harmful effects
Solution Approach 1:
The patent applies the extraction principle by selectively removing harmful components such as nitrite salts, heavy metals, and allergenic proteins from EBN through washing, enzymatic treatment, and purification steps. The beneficial peptide fragments are retained while the harmful substances are separated and discarded, reducing allergenicity while maintaining traditional EBN usage.
Solution Approach 2:
The patent converts the harmful effect of large allergenic proteins into beneficial small peptides through controlled enzymatic hydrolysis. The same enzymatic process that breaks down allergens also produces bioactive peptide fragments with therapeutic potential, transforming the harmful whole EBN into a beneficial purified extract.
3Quantity of substance
If crude EBN is used, then the raw material is readily available, but undesirable compounds such as nitrite salts, heavy metals, and adulterants are present
Solution Approach 1:
The patent applies preliminary action by performing washing, enzymatic treatment, and purification steps before the final product preparation. These preliminary steps remove nitrite salts, heavy metals, and adulterants from crude EBN, ensuring high purity is achieved before the beneficial peptides are extracted and formulated for consumption.
Solution Approach 2:
The patent uses enzymatic preparations as intermediaries to selectively bind and remove harmful substances from crude EBN. The enzymes act as mediators that differentiate between harmful components (which are removed) and beneficial proteins (which are hydrolyzed into peptides), enabling purification while maintaining availability of raw material.
4Reliability
If EBN is processed to remove contaminants, then safety is improved, but the processing complexity increases
Solution Approach 1:
The patent applies self-service by using enzymatic preparations that automatically and selectively target harmful substances in EBN. The enzymes self-organize to bind with contaminants like nitrite salts and heavy metals, and also self-hydrolyze proteins into peptides without requiring complex external control systems, thereby improving safety while minimizing processing complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method improves the bioavailability and purity of EBN extracts, reducing allergenic risks and contamination, thereby maximizing therapeutic benefits and ensuring safer, higher-quality EBN products.
Implementation Method 1
contacting the mixture with an extraction solution to bind a molecule in the mixture, wherein the extraction solution comprises at least one binding moiety selected from the group comprising an opsonin binding moiety, a complement protein binding moiety, a lectin binding moiety, a ficolin binding moiety, a collectin binding moiety, and a pentraxin binding moiety
Data Source
AI summary
The present invention relates to a method of preparing an extract from edible bird's nest, a bird's nest extract and uses of the bird's nest extract. The method comprises preparing an edible bird's nest (EBN) mixture; and contacting the mixture with an extraction solution to bind a molecule in the mixture, wherein the extraction solution comprises at least one binding moiety selected from the group comprising an opsonin binding moiety, a complement protein binding moiety, a lectin binding moiety, a ficolin binding moiety, a collectin binding moiety, and a pentraxin binding moiety.