Extracellular Matrix Body Capture via Antibody Affinity
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Solution Overview
Problem
Conventional methods for obtaining diagnostic information from biological samples are limited by the inability to detect features associated with disease, often requiring invasive biopsies and discarding valuable information from biological fluids.
Innovation Solution
The method involves isolating, separating, and enriching extracellular matrix bodies from biological fluids using immunoprecipitation techniques, allowing for the capture of these bodies on a substrate and subsequent analysis for diagnostic information.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods are used to obtain diagnostic information from biological samples, then the sample source can be closely connected to disease pathology, but invasive biopsy is required
Solution Approach 1:
The patent extracts extracellular matrix bodies from biological fluids (blood, urine, saliva) that circulate throughout the body, eliminating the need for invasive tissue biopsy. These extracted ECM bodies directly reflect disease pathology while being obtainable through simple fluid collection
Solution Approach 2:
The patent uses extracellular matrix bodies as intermediary carriers that transport disease-related information from the pathology site to accessible biological fluids. These ECM bodies serve as mediators between the disease source and the diagnostic analyst, enabling non-invasive detection
2Measurement precision
If conventional separation methods are used for biological fluids, then nucleic acids or proteins can be analyzed, but most fluid components are lost or discarded
Solution Approach 1:
The patent employs a universal separation approach using affinity capture technology that can isolate multiple different components (ECM bodies, proteins, nucleic acids) from biological fluids through a single platform, preventing loss of valuable components while enabling comprehensive analysis
Solution Approach 2:
The patent recovers and utilizes components (extracellular matrix bodies) that were previously discarded in conventional methods. By implementing affinity capture technology, the system retrieves these lost components and transforms them into valuable diagnostic information sources
3Quantity of substance
If conventional methods are used to analyze biological fluids, then common components can be detected, but obscured components are missed
Solution Approach 1:
The patent introduces affinity capture reagents (antibodies, aptamers) as intermediaries that specifically bind to and reveal obscured components in biological fluids. These intermediaries act as detectors that can identify and isolate hidden ECM bodies and other components that conventional methods miss
Solution Approach 2:
The patent changes the detection parameters by using affinity-based specific binding interactions rather than conventional bulk analysis methods. This parameter change enables the detection of low-abundance, obscured components through high-specificity molecular recognition
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach provides enhanced diagnostic analysis by directly reflecting disease pathology, reducing the need for invasive biopsies, and unlocking valuable medical information from previously overlooked components of biological fluids.
Implementation Method 1
contacting the biological fluid with the antibody to capture the extracellular matrix bodies
Data Source
AI summary
This invention relates to methods for separating, isolating and/or enriching extracellular matrix bodies in a biological fluid. More particularly, this invention discloses methods for isolating and detecting extracellular matrix bodies from a biological sample as medical information and/or for use in diagnosis and prognosis of disease. The methods include immuno-affinity capture of extracellular matrix bodies.