Ectodermal Mesenchymal Stem Cell Production for Tissue Regeneration

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing technologies have not effectively addressed the need for a specific multipotent stem cell and a method for producing the same. These are the challenges of removing elemental and oxidized mercury in existing technologies are addressed, achieving efficient and cost-effective mercury removal.

Innovation Solution

Utilization of metal sulfides (e.g., FeS2, CuS, CuFeS2) as mercury removal adsorbents, which contact with flue gas and waste liquid, adsorbing and converting Hg0 from flue gas and Hg2+ from waste liquid into stable mercury sulfide compounds.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional MSCs are continuously passaged in vitro, then cell proliferation and differentiation potency are maintained initially, but gradually lost over time

Engineering Contradiction:

Improveproliferation ability and multi-lineage differentiation potencyVSAvoidcontinuous passaging duration

Core Design Contradiction:

ReliabilityVSDuration of action of stationary object

Solution Approach 1:

The patent uses induced pluripotent stem cells (iPSCs) as a renewable source to continuously generate fresh MSCs for therapy, rather than relying on the declining functionality of continuously passaged MSCs. The iPSCs serve as a disposable reservoir that can be differentiated into MSCs on-demand, avoiding the gradual loss of proliferative and differentiation capacity that occurs with continuous in vitro passaging.

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

2Reliability

If a specific multipotent stem cell type is developed, then tissue regeneration ability is improved, but existing technologies do not effectively address the need for such specific cells

Engineering Contradiction:

Improvetissue regeneration abilityVSAvoidproduction method availability

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent performs preliminary differentiation of iPSCs into ectomesenchymal progenitor cells (EMPCs) before transplantation. This preliminary action creates a pre-conditioned cell population with enhanced regenerative capacity for specific tissues (bone, cartilage, dentin, pulp), addressing the need for tissue-specific regenerative ability while providing a manufacturable production method through controlled in vitro differentiation protocols.

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Achieves efficient, cost-effective, and environmentally friendly simultaneous removal of Hg0 from flue gas and Hg2+ from waste liquid, avoiding secondary pollution and reducing operational costs.

Implementation Method 1

metal sulfides (e.g., FeS2, CuS, CuFeS2) as mercury removal adsorbents, which contact with flue gas and waste liquid, adsorbing and converting Hg0 from flue gas and Hg2+ from waste liquid into stable mercury sulfide compounds

Methodology Applied

Scientific EffectAdsorption: Adsorption

Data Source

PatentUS12421498B2Ectodermal mesenchymal stem cells and method for producing same

Publication Date: 2025.09.23 STEMRIM INC

AI summary

The inventors discovered that ectodermal mesenchymal stem cells circulating in peripheral blood that are induced by necrotic tissue damage contribute to the regeneration of damaged tissue. On the basis of this discovery, provided are ectodermal mesenchymal stem cells, a method for producing the same, and a screening method for a substance having pluripotent stem cell inductive activity, the screening method using cells induced by necrotic tissue damage in peripheral blood as an index.