EE-GH7 Cotton Event Identification for Dual Herbicide Tolerance
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Solution Overview
Problem
Existing technologies face challenges in developing commercially viable, agronomically successful cotton plants with dual herbicide tolerance and stable genetic expression, particularly for glyphosate and HPPD inhibitor herbicides, without compromising agronomic performance.
Innovation Solution
The development of transgenic cotton plants with elite event EE-GH7, comprising specific chimeric genes and flanking sequences, allowing for rapid and accurate identification through PCR-based methods using specific primers and probes, ensuring tolerance to both glyphosate and HPPD inhibitors, and maintaining equivalent agronomic performance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic cotton plants are developed with dual herbicide tolerance genes (glyphosate and HPPD inhibitors), then herbicide tolerance is improved, but the complexity of genetic characterization and identification increases
Solution Approach 1:
The identification system is segmented into multiple specific marker sets, each targeting different aspects of the elite event EE-GH7. Instead of using a single complex identification method, the patent divides the identification task into multiple simpler PCR-based marker tests that can be performed independently and collectively confirm the presence and characteristics of the transgene insertion.
Solution Approach 2:
The patent establishes specific molecular markers and flanking sequence characteristics during the elite event selection process before commercial deployment. These pre-established markers serve as ready-to-use identification tools that simplify subsequent genetic characterization and enable rapid verification of the transgene presence without requiring complex re-analysis.
2Reliability
If extensive genetic characterization and field trials are conducted to ensure agronomic performance, then reliability of the elite event is improved, but time and resources required for development increase
Solution Approach 1:
Comprehensive genetic characterization, including flanking sequence analysis and marker establishment, is performed during the elite event selection phase before commercial release. This preliminary action ensures that all necessary verification data is collected upfront, eliminating the need for extensive post-deployment characterization and accelerating the overall development timeline.
Solution Approach 2:
The patent implements a feedback mechanism where molecular markers and genetic characteristics are continuously verified across multiple generations and field trials. This feedback loop ensures consistent agronomic performance and genetic stability, allowing for confident commercial deployment after a streamlined development process.
3Measurement precision
If specific molecular markers and flanking sequences are used for identification, then measurement precision of the elite event is improved, but the difficulty of detecting and measuring increases
Solution Approach 1:
The molecular markers and flanking sequences are designed to self-identify the elite event EE-GH7 through standard PCR amplification and sequencing procedures. The markers are inherently specific to the transgene insertion site and automatically provide precise identification without requiring complex interpretation or specialized detection equipment, making the process both precise and accessible.
Data Source
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AI summary
The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.