Egg Allergy Antigen Profiling for Precise IgE Diagnosis
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Solution Overview
Problem
Conventional allergy tests for egg allergies are inefficient due to insufficient identification of allergen components, leading to low diagnostic accuracy and reliability.
Innovation Solution
Identification of novel antigens, specifically proteins such as C-terminal portions of Vitellogenin-3 and middle or C-terminal portions of Vitellogenin-1 or Lipovitelin-1, which are used in diagnostic kits and compositions to detect IgE antibody binding, enabling precise diagnosis and treatment of egg allergies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional allergy testing agents are prepared by simply grinding candidate allergenic food, then the preparation process is simple, but the diagnostic efficiency and detection accuracy are insufficient
Solution Approach 1:
The patent extracts specific allergen components (Gal d 1, Gal d 2, Gal d 3, Gal d 4, Gal d 5) from whole egg materials to create purified antigen preparations. This extraction process isolates the diagnostically relevant components while removing interfering substances, thereby significantly improving diagnostic efficiency and detection accuracy without requiring overly complex preparation procedures
Solution Approach 2:
The patent changes the physical and chemical parameters of the antigen preparation by controlling purification conditions, concentration levels, and formulation compositions. These parameter optimizations enhance the immunoreactivity and stability of the allergen components, leading to improved diagnostic performance while maintaining practical preparability
2Reliability
If conventional allergy tests use simple grinding methods, then the manufacturing process is easy, but the patient detection rate remains far insufficient
Solution Approach 1:
The patent systematically extracts and purifies five specific egg allergen components (Gal d 1 through Gal d 5) from egg materials. This targeted extraction approach ensures comprehensive coverage of major egg allergens, significantly improving patient detection rate by identifying true positive cases that simple grinding methods miss, while the established purification protocols maintain reasonable manufacturing feasibility
Solution Approach 2:
The patent creates a multi-component antigen preparation that simultaneously targets multiple egg allergen types (ovalbumin, ovotransferrin, lysozyme C, serum albumin, and additional components). This multi-functional antigen composition enables a single diagnostic test to detect various types of egg allergies, improving overall patient detection rate while streamlining the manufacturing process through consolidated preparation methods
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel antigens allow for highly sensitive methods and kits to diagnose egg allergies, providing pharmaceutical compositions and processed egg products with reduced antigens, and testers to determine egg antigen presence, enhancing diagnostic precision and treatment efficacy.
Implementation Method 1
In serum and tissues of allergic patients, IgE antibodies specific to particular antigens (hereinafter, also referred to as 'allergens') are produced. Physiological consequences caused by interaction between such IgE antibodies and such particular antigens elicit allergic reactions.
Data Source
AI summary
The present invention provides a novel antigen for an egg allergy, a method for diagnosing an egg allergy and a kit for diagnosing an egg allergy, a pharmaceutical composition comprising the antigen, an egg, a processed product of an egg, or a bird which lays or has hatched from the egg in which the antigen is eliminated or reduced, a method for producing a processed product of an egg in which the antigen is eliminated or reduced, and a tester for determining the presence or absence of an egg antigen in an object of interest.