Egg Yolk AF-16 Protein Concentration via Tryptophan Feed
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Solution Overview
Problem
Current methods for producing egg yolks with high antisecretory factor (AF) proteins are inefficient in achieving consistent and high levels of AF protein fragments like AF-16 and AF-8, which are essential for treating abnormal physiological conditions related to excessive body fluid discharge.
Innovation Solution
A process involving feeding poultry with AF-16 or AF-8 inducing pelleted feed containing at least 0.14% free tryptophan for at least 12 weeks, followed by harvesting, separating, and processing the egg yolks through spray-drying, fluid-bed drying, grinding, leaching, extracting, evaporating, and membrane filtration to enhance the AF protein content.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional feed is used for poultry, then production cost is low, but AF protein content in egg yolk is insufficient for therapeutic use
Solution Approach 1:
The patent changes the chemical composition parameters of the poultry feed by incorporating specific precursors (tryptophan, glucose, galactose, melibiose, maltose, and/or starch) in defined quantities. This parameter modification transforms conventional feed into a specialized AF-inducing feed that produces egg yolks with therapeutically effective concentrations of AF proteins, directly resolving the contradiction between low protein content and high production cost.
2Quantity of substance
If poultry are fed AF-inducing feed for short duration, then production time is reduced, but AF protein accumulation in egg yolk is insufficient
Solution Approach 1:
The patent implements a preliminary action by feeding poultry with the specialized AF-inducing feed for a defined period (at least 4 weeks, preferably 8-12 weeks) before egg harvesting. This preliminary feeding phase allows the poultry's body to accumulate sufficient AF proteins, which are then secreted into the egg yolk, ensuring therapeutic concentrations are achieved without unnecessarily extending the production cycle.
3Quantity of substance
If egg yolk is used directly without processing, then processing steps are minimized, but AF protein concentration is too low for effective treatment
Solution Approach 1:
The patent applies extraction by separating the AF proteins from the egg yolk matrix through processing steps including heating, homogenization, and filtration. This extraction process isolates the active AF proteins (particularly AF-16 and AF-8 fragments) from other yolk components, concentrating them into a form suitable for therapeutic administration while removing unnecessary complexity of using whole egg yolk.
Solution Approach 2:
The patent creates a composite therapeutic product by combining AF proteins extracted from egg yolk with pharmaceutical excipients and formulation components. This composite material approach enhances the stability, bioavailability, and therapeutic efficacy of the AF proteins, transforming the simple egg yolk extract into an optimized pharmaceutical composition ready for clinical use.
4Reliability
If high doses of AF proteins are administered, then therapeutic effect is enhanced, but risk of side effects and immunogenicity increases
Solution Approach 1:
The patent applies local quality by providing different AF protein fragments (AF-16, AF-8, and other sequences) that may have different biological properties, stability characteristics, and immunogenicity profiles. By selecting and formulating specific fragments with optimized local properties, the patent achieves effective therapeutic doses while minimizing unwanted immune responses and side effects associated with higher dosing.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process results in egg yolks with significantly higher AF protein fragments, inducing at least 0.5 AF-Units in patients within 60-120 minutes after consumption, doubling the effectiveness compared to previous methods, and is suitable for treating conditions like diarrhea, inflammatory diseases, and glioblastoma.
Implementation Method 1
feeding a poultry, such as a hen, an AF-16 inducing pelleted feed for poultry comprising at least 0.14% free tryptophan
Implementation Method 2
spray-drying, fluid-bed drying, grinding, leaching, extracting, evaporating, membrane filtrating, and/or or freeze-drying said egg yolk
Implementation Method 3
spray-drying, fluid-bed drying, grinding, leaching, extracting, evaporating, membrane filtrating, and/or or freeze-drying said egg yolk
Implementation Method 4
spray-drying, fluid-bed drying, grinding, leaching, extracting, evaporating, membrane filtrating, and/or or freeze-drying said egg yolk
Data Source
AI summary
The present invention relates to a process for producing an egg yolk with a very high content of antisecretory factor (AF) proteins, in particular with a very high content of AF protein fragment consisting essentially of the amino acid sequence as shown in SEQ.ID.NO.1 (AF-16) and/or in SEQ.ID.NO.2 (AF-8), said process comprising for at least 4 weeks feeding a poultry, such as a hen, an AF-16 inducing pelleted feed for poultry comprising at least 0.14% free tryptophan, such as 1-2 g tryptophan/kg feed, and thereafter harvesting egg from said poultry, separating egg yolk from egg white, and alternatively spray-drying, grinding, leaching, extracting, evaporating, membrane filtrating, and/or or freeze-drying said egg yolk.The present invention further relates to said egg yolk with a very high content of antisecretory factor (AF) proteins, produced according to the herein described process, and to their use for the preparation of functional food products and pharmaceutical products for the treatment and prophylaxis of abnormal physiological conditions caused e.g. by exceptionally high levels of body fluid discharge. The invention also relates to food products and pharmaceutical products so prepared.