Solubilized Protein from Eggshell Membrane via Basic Hydrolysis
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Solution Overview
Problem
Current methods for solubilizing proteinaceous materials like eggshell membranes result in low yield, high costs, and loss of native activity, making it difficult to produce high-yield, pure, and active protein compositions on a commercial scale.
Innovation Solution
A process involving subjecting proteinaceous materials to a basic solution for hydrolysis, followed by cooling and acidification, which allows for the recovery of solubilized proteins with reduced mineral content, enabling high-yield and pure protein extraction suitable for commercial implementation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional solubilization methods (mercaptopropionic acid, extraction agents, enzymes) are used, then protein solubilization occurs, but the yield is low and costs are high
Solution Approach 1:
The patent changes the chemical parameters of the solubilization process by using basic solutions (pH 8-12) instead of conventional acidic or enzymatic methods. This parameter change enables high-yield solubilization (greater than 60% protein recovery) while reducing process complexity and cost, directly resolving the contradiction between productivity and ease of manufacture
Solution Approach 2:
The patent employs inexpensive, readily available basic solutions (such as NaOH, KOH, or ammonium hydroxide) as solubilizing agents instead of expensive enzymes or specialized extraction agents. These cheap reagents achieve superior protein recovery yields while simplifying the manufacturing process and reducing operational costs
2Productivity
If conventional solubilization methods are used, then protein extraction occurs, but native activity is lost
Solution Approach 1:
The patent carefully controls the pH parameter within a specific basic range (pH 8-12) and temperature parameters to achieve effective solubilization while preserving native protein conformation and biological activity. This parameter optimization allows high extraction efficiency without denaturing the proteins, resolving the contradiction between productivity and reliability
Solution Approach 2:
The patent performs preliminary optimization of solubilization conditions (pH, temperature, time) to establish a protocol that maximizes both protein recovery and native activity preservation. By pre-establishing optimal conditions through systematic studies, the method ensures that subsequent extractions achieve both high yield and maintained bioactivity
3Productivity
If thorough solubilization is achieved, then protein yield increases, but mineral (ash) content increases
Solution Approach 1:
The patent segments the solubilization and purification process into distinct stages: (1) basic solution treatment for protein solubilization, (2) filtration to remove insoluble mineral matter, and (3) acidification to precipitate proteins while leaving minerals in solution. This segmentation enables high protein yield while effectively separating and removing mineral contaminants
Solution Approach 2:
The patent extracts proteins from the eggshell membrane using basic solution, then separates the solubilized proteins from mineral components through filtration and selective precipitation. This extraction approach isolates the protein fraction from mineral ash, achieving both high yield and low mineral content in the final product
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process effectively solubilizes proteins from eggshell membranes with high purity and bioactivity, reducing mineral content and operational costs, making it feasible for industrial-scale applications in medical, cosmetic, and pharmaceutical industries.
Implementation Method 1
subjecting the proteinaceous material to a sufficient amount of a basic solution for a sufficient length of time and temperature for hydrolysis to occur
Implementation Method 2
cooling the mixture of proteinaceous material/basic solution
Implementation Method 3
if desired acidifying the mixture, to obtain solubilized proteins
Data Source
AI summary
The process for solubilizing proteinaceous material of the present invention includes subjecting the proteinaceous material to a sufficient amount of a basic solution to obtain a supernatant that has a basic pH and exposing the supernatant to the basic solution for a sufficient length of time and temperature for hydrolysis to occur. The process also includes cooling the mixture of the supernatant and proteinaceous material and optionally acidifying the mixture. This process may also include recovering the solubilized protein from the supernatant for use in various applications. Also provided herein is a composition of solubilized proteins from eggshell membrane obtained using processes of the present invention.