Electrode for enzymatic biosensor made from fibrous material, method for preparing same and said biosensor
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Solution Overview
Problem
Existing enzymatic biosensors face challenges with rigid three-dimensional electrodes that are difficult to agitate and expel liquid samples, limiting their usability and sensitivity due to the fixed volume of the analyte contained within the electrode.
Innovation Solution
A fibrous, compressible, and resilient electrode material that can adapt to varying liquid sample volumes, allowing for modular sampling and easy expulsion, achieved through a combination of conductive and non-conductive fibers with enzymes immobilized on specific fibers, enabling deformation and sequential measurement capabilities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If rigid three-dimensional electrodes are used, then the electrode structure is stable and maintains fixed volume, but the liquid sample is difficult to agitate and expel, limiting sensitivity and usability
Solution Approach 1:
The patent applies this principle by using a flexible membrane or thin film structure that allows the electrode to deform under external pressure. This enables easy expulsion of liquid samples while maintaining structural integrity during measurement, directly resolving the contradiction between structural stability and ease of operation.
2Device complexity
If fixed volume electrodes are used, then the electrode structure is simple, but the analyte volume is limited and cannot be modulated, reducing sensitivity
Solution Approach 1:
The patent applies this principle by making the electrode volume dynamic rather than fixed. The flexible membrane allows the electrode to expand and contract, enabling modulation of analyte volume to match different sample sizes while maintaining simple overall structure. This directly addresses the contradiction between structural simplicity and analyte quantity adaptability.
3Ease of manufacture
If enzyme-carrying fibers are mixed with conductive fibers randomly, then the electrode preparation is simple, but the interaction efficiency between enzymes and analytes is reduced
Solution Approach 1:
The patent applies this principle by creating regions with different fiber compositions within the electrode. Areas closer to the membrane surface have higher concentration of enzyme-carrying fibers to maximize interaction efficiency, while deeper regions have more conductive fibers for electron transport. This spatial differentiation resolves the contradiction between manufacturing simplicity and interaction efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The electrode facilitates efficient interaction between enzymes and analytes by applying pressure, allowing for modular liquid handling and improved sensitivity and usability in enzymatic reactions, enhancing the detection and measurement processes.
Implementation Method 1
A fibrous, compressible, and resilient electrode material that can adapt to varying liquid sample volumes
Implementation Method 2
A fibrous, compressible, and resilient electrode material that can adapt to varying liquid sample volumes
Implementation Method 3
fibers of different materials, some of which are suitable for performing the role of electrical conduction
Implementation Method 4
others are suitable for the attachment of enzymes allowing the transformation of an analyte of interest to be assayed into a measurable reaction product
Implementation Method 5
The electrode facilitates efficient interaction between enzymes and analytes by applying pressure
Data Source
Figure 1~2
Figure 3
AI summary
The present invention relates to an electrode for an enzymatic biosensor in the form of a fibrous material comprising electrically conductive and non-conductive fibers, all or part of which are functionalized with identical or different enzymes. The present invention also relates to a method for preparing such an electrode and an electrochemically sensing enzymatic biosensor comprising it.