Electromagnetic Cell Placement in Single-Cell Gel Electrophoresis

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Solution Overview

Problem

Current SCGE techniques lack automation and control in scattering cells onto the agarose layer, resulting in random and unpredictable placement, which limits analysis reliability and reproducibility.

Innovation Solution

A device utilizing electro-magnetic focusing and guidance systems to precisely control the depth and pattern of cell scattering on the agarose layer, allowing for individual cell placement and reproducible SCGE analysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If manual pipetting is used to transfer cells onto agarose layer, then the process is simple and inexpensive, but cell scattering is random and uncontrollable

Engineering Contradiction:

Improvesimplicity of cell transfer processVSAvoidcontrol over cell scattering pattern and depth

Core Design Contradiction:

Ease of manufactureVSManufacturing precision

Solution Approach 1:

The patent replaces manual mechanical pipetting with an automated system that uses electro-magnetic fields to control cell scattering. The electro-magnetic focusing system guides cells to specific locations and depths on the agarose layer, eliminating the randomness of manual placement while maintaining operational simplicity through automation.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the physical parameters of cell transfer by using electro-magnetic fields instead of mechanical force. By adjusting electro-magnetic field strength and duration, the system precisely controls cell scattering patterns and penetration depth, transforming an uncontrolled manual process into a programmable automated one.

Inventive Principle:

Principle #35Parameter changes

2Device complexity

If manual pipetting is used for cell scattering, then device complexity is low, but analysis reliability and reproducibility are limited

Engineering Contradiction:

Improvesimplicity of equipmentVSAvoidreproducibility of SCGE analysis

Core Design Contradiction:

Device complexityVSReliability

Solution Approach 1:

The patent replaces manual mechanical operations with an automated electro-magnetic system. This substitution ensures consistent and reproducible cell scattering patterns across different samples and operators, significantly improving analysis reliability while the system remains relatively compact and manageable in complexity.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The automated system performs cell scattering without requiring skilled manual intervention. The electro-magnetic focusing system automatically guides cells to predetermined positions and depths based on programmed parameters, making the process self-regulating and highly reproducible across different experimental conditions.

Inventive Principle:

Principle #25Self-service

3Productivity

If cells are scattered without automated control, then the process is fast and simple, but cell placement frequency and pattern cannot be controlled

Engineering Contradiction:

Improvespeed of cell transferVSAvoidcontrol over cell placement frequency and pattern

Core Design Contradiction:

ProductivityVSManufacturing precision

Solution Approach 1:

The patent replaces manual pipetting speed control with automated electro-magnetic guidance. The system maintains high transfer speed while precisely controlling cell placement frequency and pattern through programmable electro-magnetic field parameters, allowing rapid automated scattering with full spatial and temporal control.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent employs dynamic electro-magnetic field adjustment during the cell transfer process. The field strength, duration, and spatial distribution can be dynamically modified to control cell scattering patterns and placement frequency in real-time, enabling both high speed and precise control simultaneously.

Inventive Principle:

Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables reliable, fast, and reproducible SCGE analysis with precise control over cell placement, reducing labor and improving analysis quality by ensuring consistent cell distribution.

Implementation Method 1

This invention provides cells individually onto the LMA by hydrodynamic focusing, how deep the LMA will penetrate is determined based on the electro-magnetic guidance strength.

Methodology Applied

Scientific EffectElectro-magnetic focusing: Electromagnetic Propulsion

Implementation Method 2

by means of Electro-magnetic orientation the scattering of cell on the LMA is performed depending on the desired order

Methodology Applied

Scientific EffectElectro-magnetic orientation: Electromagnetic Propulsion

Implementation Method 3

This invention provides cells individually onto the LMA by hydrodynamic focusing

Methodology Applied

Scientific EffectHydrodynamic focusing: Fluid Spray

Data Source

PatentUS20230415143A1Single cell gel electrophoresis flow cytometry

Publication Date: 2023.12.28 TRAKYA UNIVSI REKTORLUGU

AI summary

A system ensuring the scattering of cells one by one, at a certain speed and direction, on the agarose layer on the lame in SCGE assays is provided. The system includes an electro-magnetic focusing system, a droplet nozzle moving with electro-magnetic guidance, an electric winding creating electromagnetic field for an electro-magnetic charging, an electric motor providing the movement of the lame, an electro-magnetic focusing system, and a control system controlling the electric motor.