Electrophoresis Sample Dilution Control for High-Concentration Analysis
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Solution Overview
Problem
Existing electrophoresis apparatuses face performance deterioration when samples with excessively high concentrations are analyzed, leading to inadequate separation and analysis of substances due to high concentration, surface deterioration of microchips, unexpected current flow, and concentration exceeding the normal range.
Innovation Solution
The apparatus incorporates a dilution unit to dilute samples with dilution water, utilizing a dispenser and syringe pump to mix and distribute the sample, and a control device to manage the dilution and electrophoresis processes, ensuring the concentration falls within a normal range for accurate analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If sample concentration is increased to improve detection sensitivity, then analysis sensitivity is improved, but analysis performance deteriorates due to excessive concentration
Solution Approach 1:
The system dynamically adjusts the concentration parameter of the sample by performing dilution when the concentration exceeds a predetermined threshold. The control device monitors sample concentration and automatically controls the dilution unit to add dilution water, transforming the sample from high concentration to appropriate concentration range, thereby resolving the contradiction between detection sensitivity and analysis performance
Solution Approach 2:
The control device implements a feedback mechanism by monitoring the concentration of samples and automatically triggering dilution operations when concentration exceeds the predetermined threshold. This closed-loop control ensures that samples are always within the optimal concentration range for analysis, preventing performance deterioration while maintaining detection sensitivity
2Quantity of substance
If sample concentration is excessively high, then more substance is available for analysis, but surface deterioration of microchip occurs
Solution Approach 1:
The system performs preliminary dilution of high-concentration samples before they contact the microchip surface. By detecting excessive concentration in advance and performing dilution beforehand, the system prevents the harmful effect of surface deterioration caused by high-concentration samples, allowing the microchip to operate within its safe concentration range
3Quantity of substance
If sample concentration is excessively high, then more analyte is present, but unexpected current flow occurs
Solution Approach 1:
The system changes the concentration parameter of the sample by performing dilution when the concentration exceeds the predetermined threshold. This parameter adjustment prevents high-concentration samples from causing unexpected current flow during electrophoresis, while still maintaining sufficient analyte quantity for effective analysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively prevents performance degradation by normalizing sample concentrations, allowing for precise separation and analysis of substances, even when initial concentrations are excessively high, thereby maintaining analysis quality.
Implementation Method 1
The electrophoresis unit analyzes the sample diluted by the dilution unit by electrophoresing the sample
Data Source
AI summary
An electrophoresis apparatus includes a dilution unit, an electrophoresis unit, and a control device. The dilution unit dilutes a sample with dilution water. The electrophoresis unit analyzes the sample diluted by the dilution unit by electrophoresing the sample. The control device controls the dilution unit and the electrophoresis unit.