Electrophoresis Computation Unit Corrects Spectrum
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Solution Overview
Problem
Current electrophoresis methods require repeated spectral calibration when electrophoresis conditions or fluorescent dyes change, leading to increased labor and costs due to deviations between reference and actual sample spectra, resulting in erroneous fluorescence strength measurements and pseudo peaks.
Innovation Solution
An electrophoresis device with a computation unit that corrects spectra using pre-determined correction factors for each electrophoresis condition or fluorescent dye, ensuring accurate fluorescence strength calculation without the need for repeated spectral calibration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If spectral calibration is performed for each electrophoresis condition or fluorescent dye change, then measurement precision is improved, but loss of time and productivity deteriorate due to repeated calibration operations
Solution Approach 1:
The patent applies preliminary action by pre-calculating and storing correction factors for various electrophoresis conditions and fluorescent dyes before actual measurement. When a specific condition or dye is selected, the corresponding pre-computed correction factor is directly applied without requiring real-time spectral calibration, thus eliminating time loss while maintaining measurement precision.
Solution Approach 2:
The patent uses parameter changes by creating a library of correction factors corresponding to different electrophoresis conditions (voltage, temperature, buffer composition) and fluorescent dyes. By identifying the current parameters and selecting the matching pre-computed correction factor, the system avoids repeated calibration while adapting to different measurement conditions.
2Reliability
If spectral calibration is repeated for each condition change, then reliability is improved by preventing pseudo peaks, but device complexity increases due to multiple calibration procedures
Solution Approach 1:
The patent resolves the complexity issue by performing the complex spectral calibration work in advance and storing the results as correction factors. The actual measurement process simply retrieves and applies the appropriate pre-computed factor, maintaining reliability by preventing pseudo peaks while dramatically simplifying the operational procedure.
Solution Approach 2:
The patent applies copying by creating a library of correction factors that represent the calibrated spectral characteristics for different conditions and dyes. Instead of performing complex calibration procedures repeatedly, the system copies and applies the appropriate pre-established correction pattern, maintaining accuracy while reducing procedural complexity.
3Measurement precision
If operators perform manual spectral calibration, then measurement precision is maintained, but labor costs and operational complexity increase
Solution Approach 1:
The patent implements self-service by automating the selection and application of correction factors based on the detected electrophoresis conditions and fluorescent dye type. The system automatically identifies the appropriate pre-computed correction factor and applies it without requiring operator intervention in the calibration process, maintaining precision while greatly improving ease of operation.
Solution Approach 2:
The patent uses feedback by continuously monitoring the electrophoresis conditions and fluorescent dye identification, then automatically selecting and applying the corresponding correction factor from the library. This closed-loop approach ensures measurement precision is maintained while eliminating manual calibration operations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces operator labor and costs by maintaining accurate fluorescence strength measurements across varying conditions, eliminating pseudo peaks and ensuring consistent results without repeated calibration.
Implementation Method 1
a dispersion element for dispersing light from the sample within the electrophoresis path
Implementation Method 2
a photodetector for detecting the light dispersed by the dispersion element
Implementation Method 3
Each DNA is labelled by a fluorescent dye, and fluorescence is produced by irradiation of excitation light
Implementation Method 4
the computation unit corrects the spectrum using correction factors determined for each electrophoresis condition or fluorescent dye
Data Source
AI summary
An electrophoresis device of the present disclosure includes an electrophoresis path of a sample, a dispersion element for dispersing light from the sample within the electrophoresis path, a photodetector for detecting the light dispersed by the dispersion element, and a computation unit for determining a spectrum of the light on the basis of a signal from the photodetector, and is characterized in that the computation unit corrects the spectrum using correction factors determined for each migration condition or fluorescent dye.