Electrospun Sheet for Light Emission Detection in Reaction Fields

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Solution Overview

Problem

Conventional pathological diagnoses are reliant on techniques and experience, leading to variability and inaccuracies, especially in determining cell characteristics for personalized cancer treatments, and molecular pathological examination procedures face challenges due to indefinite target cell content.

Innovation Solution

An examination device with a detection unit, a light-emitting container, and a sheet member formed by electrospinning, which allows for the culturing of cells and visualization of their characteristics through optical properties, enabling accurate detection of target cells based on light emission reactions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional pathological diagnosis methods are used, then operator experience and technique are required for accurate diagnosis, but this leads to variability and inaccuracy in determining cell characteristics

Engineering Contradiction:

Improvediagnostic accuracyVSAvoidreliance on operator expertise

Core Design Contradiction:

Measurement precisionVSEase of operation

Solution Approach 1:

The patent replaces manual pathological examination with an automated optical detection system. The detection unit with light source and sensor automatically analyzes cell characteristics through light emission reactions, eliminating dependence on operator experience and technique while maintaining or improving diagnostic accuracy.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The examination system performs self-diagnosis through automated detection. The detection unit independently identifies target cells by measuring light emission from reactions within cells, without requiring external operator intervention or interpretation, thereby reducing variability and improving consistency in diagnostic results.

Inventive Principle:

Principle #25Self-service

2Reliability

If molecular pathological examination procedures (FACS, FISH, PCR) are used, then auxiliary diagnostic capabilities are improved, but decision turnovers occur due to indefinite target cell content

Engineering Contradiction:

Improvediagnostic consistencyVSAvoidindefinite target cell content

Core Design Contradiction:

ReliabilityVSLoss of information

Solution Approach 1:

The patent utilizes light emission (optical signal) as a detectable indicator of cell characteristics. By measuring the intensity and properties of light emitted from cellular reactions, the system objectively quantifies target cell content, eliminating the indefiniteness that causes decision turnovers in molecular examination procedures.

Inventive Principle:

Principle #32Color changes

Solution Approach 2:

The patent replaces subjective interpretation of molecular examination results with objective optical measurement. The detection unit quantitatively measures light emission from cellular reactions, providing clear, unambiguous data that eliminates decision turnovers and improves diagnostic consistency.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If traditional cell examination methods are used, then cell characteristics can be observed, but the intake ratio of target cells into the examination system is low

Engineering Contradiction:

Improvecell intake ratioVSAvoidnumber of target cells examined

Core Design Contradiction:

ProductivityVSQuantity of substance

Solution Approach 1:

The patent changes the detection parameter from requiring large numbers of cells to detecting light emission from individual or small groups of cells. By using optical detection that can identify target cells through their light emission properties, the system achieves high cell intake ratios while examining fewer total cells, improving productivity.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The device enhances diagnostic accuracy by providing a high-sensitivity detection method that improves cell intake ratios and allows for real-time visualization of cell activity, reducing reliance on operator expertise and improving treatment policy decisions.

Implementation Method 1

a sheet capable of adsorbing and gradually releasing the reagent

Methodology Applied

Scientific EffectAdsorption: Adsorption

Implementation Method 2

causing light emission by a reaction between a reagent and a measurement target

Methodology Applied

Scientific EffectChemiluminescence: Chemiluminescence

Implementation Method 3

causing light emission by a reaction between a reagent and a measurement target in a reaction field

Methodology Applied

Scientific EffectBioluminescence: Bioluminescence

Data Source

PatentUS20240247223A1Examination method of a measurement target by light emitted in a reaction field

Publication Date: 2024.07.25 KK TOSHIBA

AI summary

Provided is an examination method wherein a measurement target is produced in a reaction field within a container and reacted with a reagent to cause light emission which is received, and the optical characteristics thereof detected by a detection unit. The reaction field contains a plurality of pieces in a solution in which the reagent dissolves, the pieces being capable of adsorbing the reagent and gradually releasing the adsorbed reagent for reaction with the measurement target. The container is arranged on the detection unit such that the detection unit faces the reaction field.