Elongated Frit Chromatography Column for High-Volume Biomolecule Separation
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Solution Overview
Problem
Conventional chromatographic columns face limitations in processing large sample volumes due to increased backpressure, which restricts the flow rate and efficiency of sample processing, especially in automated liquid processing systems.
Innovation Solution
A separation device with a column design featuring an elongated frit arranged parallel to the column side wall, allowing for perpendicular and axial liquid flow, which disperses the separation media efficiently and reduces backpressure, enabling faster processing of larger sample volumes without clogging or losing liquid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If the bed volume of the chromatographic column is increased to process larger sample volumes, then the sample processing capacity is improved, but the backpressure increases dramatically which slows the flow rate
Solution Approach 1:
The frit is segmented into an elongated structure extending parallel to the column side wall, creating multiple flow pathways that distribute the liquid flow and reduce backpressure while maintaining the ability to process large sample volumes
Solution Approach 2:
The frit is arranged in a parallel orientation rather than the conventional perpendicular arrangement, extending along the length of the column to create a two-dimensional flow distribution system that reduces resistance to flow
2Quantity of substance
If the bed volume of the chromatographic column is increased to process larger sample volumes, then the sample processing capacity is improved, but the chamber space above the upper frit is limited
Solution Approach 1:
The frit is divided into an elongated structure that segments the column space, allowing for increased bed volume while maintaining adequate chamber space above the frit for proper liquid flow and sample processing
3Reliability
If an intermediate porous barrier is added to prevent sorbent from passing through, then the sorbent containment is improved, but backpressure increases and liquid solution recovery decreases
Solution Approach 1:
The frit itself is a porous material that serves as the barrier to sorbent while maintaining low backpressure through its elongated structure that provides multiple flow pathways, eliminating the need for additional intermediate porous barriers
Solution Approach 2:
The frit combines the functions of sorbent containment and flow distribution in a single composite structure, integrating the barrier function with the flow management function to avoid the drawbacks of separate porous barriers
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device allows for efficient separation and processing of up to 40 mL of sample in one stroke with reduced backpressure, enhancing the capture and recovery of biomolecules like plasmids and viruses, and supports dual chromatography flow for improved sample processing.
Implementation Method 1
at least part of an elongated frit has been arranged substantially parallel to the column side wall segregating separation media from the bottom liquid inlet/outlet
Implementation Method 2
Chromatographic columns may be constructed with a packed bed of media
Implementation Method 3
In the area of chromatography, it is known that larger bed volumes limit the amount a chamber space above the upper frit
Data Source
AI summary
The present invention relates to the readily automated methods for the separation of biomolecules, such as proteins or nucleic acids. More specifically, the invention relates to a separation device, which includes at least one column provided with a bottom liquid inlet/outlet and adapted to receive a separation media. At least part of an elongated frit has been arranged substantially parallel to the column side walls, segregating the separation media from the bottom liquid inlet/outlet. The elongated frit may be shaped as a tube and should have a porosity that serves to retain separation media within the column during sample processing.Further, the invention relates to a method of separating at least one target molecule from a liquid, which method comprises providing at least one column provided with a bottom liquid inlet/outlet and optionally with a top liquid inlet. The column retains a separation media; and at least part of an elongated frit has been arranged substantially parallel to the column side walls to segregate the separation media from the bottom liquid inlet/outlet. A liquid sample is processed in said column in a method including aspiration and dispensing of fluid to and from the bottom liquid inlet/outlet; adding a liquid at the top liquid inlet; or any combination thereof.