Embryo Culture Dish Layout for Noninvasive Chromosome Sampling
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Solution Overview
Problem
Current embryo culture devices face challenges in efficiently sampling culture solution for chromosome analysis without disrupting the culture environment, leading to increased distances between wells, reduced imaging capacity, and higher transfer errors.
Innovation Solution
An embryo culture dish with adjacent wells featuring recess parts and movement block parts to maintain solution circulation while preventing embryo movement, allowing for efficient sampling and imaging without disturbing the culture environment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If the volume of a well that contains an embryo is increased to allow sufficient culture solution for testing, then a necessary amount of culture solution can be sampled for chromosome analysis, but the distance between adjacent wells increases, reducing the number of wells that can be imaged at once and complicating the photographing device structure
Solution Approach 1:
The well is segmented into two functional zones: a culture area for the embryo and a collection area for the culture solution. This segmentation allows the well to accommodate both the embryo and sufficient culture solution volume for testing without increasing the overall well diameter, thereby maintaining compatibility with existing photographing devices.
Solution Approach 2:
The collection area is formed as a recess below the culture area, utilizing the vertical dimension rather than increasing the horizontal diameter. This dimensional transition allows sufficient culture solution volume to be contained within the same footprint, maintaining well density for imaging while enabling adequate sampling volume.
2Quantity of substance
If the volume of a well that contains an embryo is increased to allow sufficient culture solution for testing, then a necessary amount of culture solution can be sampled for chromosome analysis, but the distance between adjacent wells increases, increasing the distance for transferring embryos and the probability of mistakes
Solution Approach 1:
The well is segmented into two functional zones: a culture area for the embryo and a collection area for the culture solution. This segmentation allows the well to accommodate both the embryo and sufficient culture solution volume for testing without increasing the overall well diameter, thereby maintaining compatibility with existing photographing devices.
Solution Approach 2:
The collection area is formed as a recess below the culture area, utilizing the vertical dimension rather than increasing the horizontal diameter. This dimensional transition allows sufficient culture solution volume to be contained within the same footprint, maintaining well density for imaging while enabling adequate sampling volume.
3Quantity of substance
If a necessary amount of culture solution is sampled from microwells with small diameter, then chromosome analysis can be performed, but the embryo culture environment greatly changes
Solution Approach 1:
The collection area is designed as a separate recess that can be selectively accessed for sampling. This allows the culture solution in the collection area to be removed for testing without disturbing the embryo in the culture area, thereby maintaining the stability of the embryo culture environment while obtaining sufficient sample volume.
Solution Approach 2:
The well is segmented into two functional zones: a culture area for the embryo and a collection area for the culture solution. This segmentation allows the well to accommodate both the embryo and sufficient culture solution volume for testing without increasing the overall well diameter, thereby maintaining compatibility with existing photographing devices.
Data Source
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AI summary
In an embryo culture dish that includes a plurality of wells that each contain an embryo together with a culture solution, the plurality of wells are disposed independently and adjacently at a bottom part of the embryo culture dish, a recess part that can retain the culture solution together with the well is formed per well at the bottom part independently from another recess part, a movement block part that permits circulation of the culture solution and blocks movement of the embryo is provided between the well and the recess part, and a volume of the recess part is equal to or more than a volume of a culture solution to be sucked up for testing for aneuploidy of a chromosome of the embryo cultured in the well. By so doing, it is possible to suck up the culture solution for testing for aneuploidy of chromosomes of an embryo to be cultured, and dispose the wells close to each other that contain a plurality of embryos.