Electrochemistry Chip Layout for Stable Embryo OCR Detection
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Solution Overview
Problem
Existing electrochemistry detection chips face issues with reference potential stability and specimen fixing, leading to inefficient embryo OCR measurements.
Innovation Solution
The electrochemistry detection chip incorporates a reference material layer composed of silver chloride (AgCl), a first structural layer with support arms, and a covering layer with separate working and reference wells connected by a salt bridge, enhancing stability and reducing fluid intermixing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a single-thickness microwell is used to fix the embryo, then the device structure is simple, but the specimen fixing stability is insufficient
Solution Approach 1:
The single-thickness microwell is divided into a first structural layer and a second structural layer, with the first layer providing support arms for specimen positioning and the second layer forming the actual microwell cavity. This segmentation allows independent optimization of fixing stability and device simplicity.
2Device complexity
If the reference electrode and working electrode are in the same well, then the device structure is simple, but the reference potential stability deteriorates due to fluid intermixing
Solution Approach 1:
The single well is segmented into a first well containing the reference electrode and a second well containing the working electrode, connected by a salt bridge. This spatial segmentation prevents fluid intermixing while maintaining electrical connectivity, resolving the contradiction between structural simplicity and potential stability.
Solution Approach 2:
A salt bridge is introduced as an intermediary element connecting the reference well and working well. The salt bridge allows ionic conduction between the two wells while preventing direct fluid mixing, thus maintaining reference potential stability without significantly increasing device complexity.
3Device complexity
If AgCl reference material is applied directly on the reference electrode in the working well, then the device structure is simple, but harmful effects occur due to fluid intermixing
Solution Approach 1:
The reference well and working well are segmented and separated by a salt bridge, allowing AgCl reference material to be applied in the reference well without direct contact with the specimen in the working well. This eliminates harmful effects while maintaining the simplicity of using AgCl as reference material.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This design stabilizes the reference potential, improves specimen fixing, and increases detection efficiency by minimizing fluid leakage and vortex formation during embryo placement.
Implementation Method 1
a salt bridge connecting layer which is disposed on the base layer, and one end of it is located in the working well, and the other end of it is located in the reference well
Implementation Method 2
a reference material layer which is disposed on the reference electrode and is composed of silver chloride (AgCl)
Data Source
AI summary
An electrochemistry detection chip which comprises: a base layer, an electrode chip, an insulating layer, a reference material layer, a first structural layer which is disposed on the insulating layer and comprises: a plurality of bottom support arms, wherein the ends of the bottom support arms are adjacent to each other to jointly define a specimen accommodation area; a covering layer, and a salt bridge connecting layer. The electrochemistry detection chip of the present invention can be used to detect the respiratory activity of embryos.