Filtering Device for Embryo Suspension Mass Separation
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Solution Overview
Problem
Current methods for separating embryo suspension mass (ESM) are prone to contamination and cause shearing and compaction damage to cells, particularly during density-based centrifugation, and are not cost-effective.
Innovation Solution
A filtering device is used to suspend the ESM in a fluid and force the mixture through a filter with pores of specific diameters to separate particles of different sizes, minimizing damage and contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If density-based centrifugation is used for size separation, then separation effectiveness is improved, but cell damage (shearing and compaction) increases
Solution Approach 1:
The patent replaces the mechanical centrifugal force system with a filtration system that uses pressure differential to achieve separation. Instead of using centrifugation which causes shearing and compaction damage, the invention uses a filter with specific pore sizes to separate cells based on size while maintaining cell integrity through gentler pressure-driven filtration.
Solution Approach 2:
The patent introduces a filtration system as an intermediary between the cell suspension and the separation process. The filter acts as a mediator that separates cells by size without directly subjecting them to the harmful mechanical forces of centrifugation, thereby reducing cell damage while achieving effective separation.
2Measurement precision
If conventional size separation methods are used, then separation capability is improved, but contamination risk increases
Solution Approach 1:
The patent employs disposable filters that can be easily replaced or discarded after use. This approach prevents cross-contamination between different cell cultures by ensuring that each filtering operation uses a fresh, sterile filter, thereby eliminating the contamination risk associated with reusable separation equipment.
3Measurement precision
If sieve or nested sieve methods are used for size separation, then separation function is achieved, but operational complexity and cost increase
Solution Approach 1:
The patent extracts the essential separation function from complex multi-stage sieve systems and implements it through a single filter with specifically engineered pore sizes. This simplifies the operational process by eliminating the need for multiple nested sieves and complex procedural steps while maintaining effective size-based separation capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively separates ESM particles while reducing the risk of damage and contamination, achieving a higher yield of healthy plants and trees by maintaining cell integrity and reducing costs.
Implementation Method 1
forcing the mixture through a filter with pores of specific diameters to separate particles of different sizes
Data Source
AI summary
A method of separating an embryo suspension mass is provided. The method includes supplying an embryo suspension mass culture having a plurality of first particles of a first size and a plurality of second particles of a second size different at least in part from the first size of the first particles. The method also includes suspending the embryo suspension mass culture in a fluid to create a mixture and forcing the mixture through a filter while maintaining the mixture in the fluid to separate the first particles from the second particles.