Embryonic Membrane Supernatant for Serum-Free Animal Cell Growth
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Solution Overview
Problem
Existing animal cell culture media lack effective and novel growth promoters beyond traditional sera, hormones, and growth factors, limiting the efficiency and diversity of cell culture methods.
Innovation Solution
Utilizing a culture supernatant derived from the embryonic membrane of avian or reptilian eggs, such as chicken eggs, as an active ingredient in animal cell growth promoters, and employing a device with controlled medium circulation for optimal cell culture conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If fetal bovine serum or hormones are used as growth factors in culture media, then cell growth is maintained, but the cost and complexity of the culture system increase
Solution Approach 1:
The invention extracts and utilizes the growth-promoting activity from embryonic membranes through culture supernatant, isolating the essential growth factor function from complex serum compositions. This extraction approach maintains cell growth while simplifying the overall culture system by replacing complex serum mixtures with a targeted biological extract.
Solution Approach 2:
The culture supernatant from embryonic membranes serves as a cost-effective alternative to expensive fetal bovine serum. By using a renewable biological source (embryonic membranes) that can be cultured and harvested, the system replaces costly, complex serum products with a more economical growth promoter.
2Reliability
If conventional culture media with serum are used, then cell growth is supported, but novel growth promoters and alternatives are limited
Solution Approach 1:
The invention introduces culture supernatant from embryonic membranes as an intermediary growth promoter that bridges the gap between conventional serum-based systems and the need for novel alternatives. This intermediary substance provides growth support while representing a new class of growth promoters distinct from traditional serum, hormones, or plant extracts.
3Ease of operation
If simple culture vessels are used, then the system is easy to operate, but controlled medium circulation and optimal cell propagation conditions cannot be achieved
Solution Approach 1:
The culture device incorporates dynamic medium circulation through pumps and flow paths, transforming a static culture system into an active, controlled system. The medium-flow-rate control unit dynamically adjusts flow conditions to optimize cell propagation, demonstrating how controlled dynamics can enhance productivity while maintaining operational simplicity through automation.
Solution Approach 2:
The medium-flow-rate control unit implements feedback control by monitoring culture medium conditions and adjusting flow rates accordingly. This feedback mechanism ensures optimal cell propagation conditions are maintained automatically, enhancing productivity while reducing the need for manual intervention and maintaining ease of operation.
Data Source
AI summary
The present invention aim at providing a novel animal cell growth promoter. Specifically, a culture supernatant of an embryonic membrane derived from an avian or reptilian fertilized egg is use as an animal cell growth promoter. For example, by adding, to an animal cell culture medium, an animal cell growth promoter comprising, as an active ingredient, a culture supernatant of an embryonic membrane derived from an avian or reptilian fertilized egg, the animal cell growth can be promoted.