Encapsulated Cells Cryopreservation for Tunable Therapeutic Delivery

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Solution Overview

Problem

Current biomedical therapies for diseases like cancer often have limited patient responsiveness and lack controlled delivery mechanisms for therapeutic agents, necessitating the development of implantable devices for localized and tunable administration.

Innovation Solution

Methods for cryopreserving populations of encapsulated cells, which include contacting the cells with a cryopreservation media, optionally incubating at room temperature, and cooling to preserve cell viability, along with techniques for maintaining and thawing these cells to ensure their functionality for controlled antigen or therapeutic agent delivery.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If current biomedical therapies are used for localized and targeted treatment, then therapeutic delivery is improved, but patient responsiveness remains modest and control over delivery timing is limited

Engineering Contradiction:

Improvetherapeutic delivery effectivenessVSAvoidcontrol over delivery timing

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent employs dynamic control of therapeutic agent delivery through implantable devices that can adjust release rates and timing. The system transitions from static, fixed-delivery therapies to dynamic, controllable delivery mechanisms that respond to physiological conditions and allow clinicians to modulate therapeutic output over time

Inventive Principle:

Principle #15Dynamics

Solution Approach 2:

The invention utilizes parameter changes in the form of tunable delivery devices that can modify release kinetics, concentration gradients, and temporal patterns of therapeutic agent administration. This allows optimization of delivery parameters to match patient-specific requirements and disease progression stages

Inventive Principle:

Principle #35Parameter changes

2Duration of action of stationary object

If encapsulated cells are cryopreserved using conventional methods, then storage is achieved, but cell viability and functionality may be compromised

Engineering Contradiction:

Improvestorage durationVSAvoidcell viability

Core Design Contradiction:

Duration of action of stationary objectVSReliability

Solution Approach 1:

The patent applies controlled phase transition principles in cryopreservation by managing the transition from liquid to solid state through controlled cooling rates and temperature gradients. The system navigates through critical temperature zones to minimize ice crystal formation and cellular damage while achieving long-term stable storage

Inventive Principle:

Principle #36Phase transitions

Solution Approach 2:

The invention uses cryoprotectant agents as intermediary substances that mediate between the cellular environment and freezing conditions. These intermediaries protect cells during the phase transition by reducing ice crystal formation, stabilizing membranes, and maintaining cellular integrity throughout the cryopreservation process

Inventive Principle:

Principle #24Intermediary (Mediator)

3Ease of operation

If encapsulated cells are used for therapeutic delivery, then localized administration is achieved, but immunoreactivity may occur

Engineering Contradiction:

Improvelocalized administrationVSAvoidimmunoreactivity

Core Design Contradiction:

Ease of operationVSObject-affected harmful factors

Solution Approach 1:

The patent employs encapsulation shells as flexible barriers that physically isolate therapeutic cells from the host immune system while allowing selective permeability for nutrient exchange and therapeutic product delivery. The shell acts as a protective interface that maintains localized administration while reducing immunogenic recognition

Inventive Principle:

Principle #30Flexible shells and thin films

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The methods effectively preserve and maintain the viability of encapsulated cells, enabling controlled release of therapeutic agents, reducing immunoreactivity, and providing a tunable delivery mechanism for targeted therapies.

Implementation Method 1

contacting the encapsulated cells with an effective amount of a cryopreservation media to form a cryopreservation composition; cooling the cryopreservation composition to a temperature sufficient to cryopreserve the encapsulated cells

Methodology Applied

Scientific EffectCryopreservation: Freezing

Implementation Method 2

The encapsulated cells described herein comprise a zone or layer that encapsulates or admixes with the antigenic target, preventing contact of a host immune effector cell with the antigenic target

Methodology Applied

Scientific EffectDiffusion: Diffusion

Data Source

PatentUS20230320346A1Encapsulated cells and methods of preserving thereof

Publication Date: 2023.10.12 AVENGE BIO INC

AI summary

The present disclosure relates to methods of preserving encapsulated cells and related compositions and methods of using the same.