Endocide-Mediated Plant Mutation and Growth Control

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Solution Overview

Problem

Current methods for inducing mutations in plants are non-specific and often result in broad toxicity, lacking the ability to selectively modify growth in target species without affecting non-closely related species, and there is a need for technologies that can develop new plant varieties through pruning and mutation induction.

Innovation Solution

The use of endocides, such as 4-hydroxybenzoic acid and 3,4-dihydroxybenzoic acid, derived from specific species or their closely related species, to modify plant growth through pruning, decapitation, fragmentation, and prolonged soaking of propagules in water, allowing for selective induction of mutations in target species.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional mutagenesis methods are used to induce mutations in plants, then mutations can be induced, but the methods lack species specificity and cause broad toxicity affecting non-target species

Engineering Contradiction:
Improvespecies specificity of mutation inductionVSAvoidbroad toxicity to non-target species
Core Design Contradiction:
Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The patent uses species-specific secondary metabolites (endocides) as intermediary substances that mediate between the treatment application and the target plant species. These endocides act as selective mediators that trigger endogenous defense responses and mutations only in the target species through species-specific recognition mechanisms, while leaving non-target species unaffected.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent changes the chemical parameter of using species-specific secondary metabolites with distinct chemical structures and biological activities. By selecting endocides unique to or predominant in the target species, the method achieves parameter-based differentiation that enables selective mutation induction without broad toxicity.

Inventive Principle:
Principle #35Parameter changes

2Productivity

If pruning techniques are applied to promote lateral growth, then lateral growth is enhanced, but there is no reported technology to develop new plant varieties through pruning-induced mutations

Engineering Contradiction:
Improvelateral growth promotionVSAvoidability to develop new plant varieties
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent merges two previously separate functions: pruning (which promotes lateral growth) and mutagenesis (which induces mutations). By applying species-specific endocides to pruned plants, the method combines growth promotion with variety development, creating a unified technique that achieves both lateral growth enhancement and new variety creation simultaneously.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent applies pruning as a preliminary action before endocide treatment. The pruning creates physiological stress and activates defense mechanisms in advance, making the plant more responsive to subsequent endocide application. This preliminary structural modification prepares the plant for enhanced mutation induction while promoting lateral growth.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enables the selective induction of mutations in target species with minimal impact on non-closely related species, resulting in new plant varieties with desired morphological and chemical characteristics, such as shrubbiness, dwarfism, and altered chemical biosynthesis.

Implementation Method 1

compositions and methods for inducing mutations in organisms

Methodology Applied
Scientific EffectMutation induction:

Implementation Method 2

prolonged soaking of propagules in water

Methodology Applied
Scientific EffectAbsorption: Absorption (physical)

Data Source

PatentUS11350582B2Plant varieties by application of endocides
Publication Date: 2022.06.07 STEPHEN F AUSTIN STATE UNIV
  • US11350582B2 patent drawing
  • US11350582B2 patent drawing
  • US11350582B2 patent drawing

AI summary

The present invention relates generally to compositions and methods for mutating a plant and plants and plant products produced by said methods. Also, compositions and methods for controlling a plant species are disclosed herein.