Endodermal Progenitor Cell Isolation Without Stressors

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Solution Overview

Problem

Current methods for isolating and culturing pancreatic progenitor cells require exogenous stressors, which reduces their clinical applicability due to potential tumorigenic transformation and functional impairment.

Innovation Solution

A method for isolating endodermal progenitor cells from tissues like the pancreas without subjecting them to exogenous stressors, involving culturing cell populations to form clusters in the presence of attachment factors and specific growth factors, allowing for the isolation and differentiation of these cells without chemical or physical stress.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If exogenous stressors are applied to isolate pancreatic progenitor cells, then isolation can be achieved, but cell viability and clinical applicability are reduced due to potential tumorigenic transformation and functional impairment

Engineering Contradiction:

Improveisolation capabilityVSAvoidcell viability

Core Design Contradiction:

Ease of manufactureVSReliability

Solution Approach 1:

The patent extracts and eliminates the harmful exogenous stressors (chemicals, physical stressors) from the isolation process while maintaining the ability to isolate progenitor cells through alternative methods that do not compromise cell viability or induce tumorigenic transformation

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent converts the harmful effect of exogenous stressors into a beneficial isolation method by using physiological conditions and natural cell behaviors to achieve separation without damage, thereby preserving cell function and preventing tumorigenesis

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

2Productivity

If exogenous stressors are used to culture pancreatic progenitor cells, then cell proliferation can be induced, but functional impairment and tumorigenic transformation occur

Engineering Contradiction:

Improvecell proliferationVSAvoidtumorigenic transformation

Core Design Contradiction:

ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the cultural parameters from exogenous stressor-based induction to physiological condition-based proliferation, using natural growth factors and optimal environmental conditions to induce cell division without tumorigenic transformation

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent introduces intermediary substances such as growth factors and cytokines that mediate cell proliferation through natural signaling pathways, avoiding the direct harmful effects of exogenous stressors while maintaining productive cell expansion

Inventive Principle:

Principle #24Intermediary (Mediator)

3Ease of manufacture

If traditional isolation methods are applied, then progenitor cells can be obtained, but the cells require exogenous stressors that reduce their clinical utility

Engineering Contradiction:

Improveisolation processVSAvoidclinical applicability

Core Design Contradiction:

Ease of manufactureVSAdaptability or versatility

Solution Approach 1:

The patent enables progenitor cells to undergo isolation and proliferation through self-service mechanisms, utilizing their intrinsic biological properties and natural behaviors to separate and expand without requiring harmful exogenous stressors, thereby preserving their clinical applicability

Inventive Principle:

Principle #25Self-service

Data Source

PatentUS9005964B2Endodermal progenitor cells

Publication Date: 2015.04.14 HEALIOS KK

AI summary

The present invention relates to endodermal progenitor cells and methods of isolation, culture, differentiation and use thereof.