Endophyte Seed Coating for Crop Yield and Stress Tolerance

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Solution Overview

Problem

Current agricultural practices face challenges in increasing food production to meet the growing global population due to scarce resources and climate changes, with existing methods like genetically modified crops and synthetic chemicals facing acceptance issues and limited effectiveness in improving crop yield and resilience to environmental stresses.

Innovation Solution

The use of novel compositions of bacterial and fungal endophytes that are conserved across diverse plant species, applied to seeds or plant elements to enhance agronomic traits such as yield, disease resistance, and stress tolerance, by coating the seeds with purified microbial populations capable of metabolizing specific substrates and exhibiting functions like auxin production and nitrogen fixation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If genetically modified crops and synthetic chemicals are used to improve crop yield and resilience, then agricultural productivity increases, but public acceptance decreases and regulatory barriers increase

Engineering Contradiction:
Improvecrop yieldVSAvoidpublic acceptance
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

The patent uses beneficial microorganisms (endophytes and rhizosphere bacteria) as intermediary agents to mediate between the crop and the environment. These microorganisms naturally enhance crop yield and stress resistance through biological mechanisms such as nitrogen fixation, phosphate solubilization, and production of plant growth-promoting substances, avoiding the need for genetically modified organisms or synthetic chemicals that face public resistance

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent employs self-service mechanisms where beneficial microorganisms naturally colonize the crop roots and tissues, providing ongoing services such as nutrient acquisition, pathogen protection, and stress tolerance. The microorganisms reproduce and persist in the rhizosphere and plant tissues, continuously delivering agronomic benefits without requiring repeated external applications of synthetic chemicals

Inventive Principle:
Principle #25Self-service

2Productivity

If conventional agricultural methods are used to meet growing food demand, then food production increases, but resource depletion and environmental degradation worsen

Engineering Contradiction:
Improvefood productionVSAvoidresource depletion
Core Design Contradiction:
ProductivityVSLoss of substance

Solution Approach 1:

The beneficial microorganisms perform self-service functions by naturally fixing atmospheric nitrogen, solubilizing insoluble phosphates, and producing plant growth-promoting substances. This reduces or eliminates the need for synthetic nitrogen and phosphate fertilizers, preventing nutrient runoff and groundwater contamination while maintaining high crop yields

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The microorganisms act as intermediaries that convert unavailable nutrients (atmospheric nitrogen, rock phosphate) into plant-available forms through biological processes. This natural nutrient cycling reduces dependence on energy-intensive synthetic fertilizer production and minimizes environmental pollution from fertilizer runoff

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If crop yields are increased to feed growing population, then food security improves, but resilience to environmental stresses decreases

Engineering Contradiction:
Improvecrop yieldVSAvoidstress tolerance
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The beneficial microorganisms provide ongoing protective services by producing antimicrobial compounds that suppress soil-borne pathogens, generating plant growth-promoting hormones that enhance stress tolerance, and improving water and nutrient uptake efficiency. This dual enhancement of yield and stress resistance occurs through the microorganisms' continuous biological activities in the rhizosphere and plant tissues

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent applies beneficial microorganisms to seeds or soil before crop establishment, allowing the microorganisms to colonize the rhizosphere and plant tissues early in development. This preliminary colonization establishes protective and promotive relationships before environmental stresses or pathogens become problematic, enhancing both yield potential and stress resilience throughout the crop lifecycle

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The application of these endophytes leads to significant improvements in crop yield, resilience to environmental stresses, and enhanced agronomic traits, overcoming the limitations of previous methods by efficiently colonizing plant tissues and conferring multiple beneficial properties.

Implementation Method 1

capable of metabolizing specific substrates and exhibiting functions like auxin production and nitrogen fixation

Methodology Applied
Scientific EffectMetabolism: Fermentation

Implementation Method 2

exhibiting functions like auxin production and nitrogen fixation

Methodology Applied
Scientific EffectNitrogen fixation: Nitriding

Implementation Method 3

exhibiting functions like auxin production and nitrogen fixation

Methodology Applied
Scientific EffectAuxin production:

Data Source

PatentUS11570993B2Endophytes, associated compositions, and methods of use
Publication Date: 2023.02.07 INDIGO AG INC
  • US11570993B2 patent drawing
  • US11570993B2 patent drawing
  • US11570993B2 patent drawing

AI summary

Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.