Endotoxin Visualization System Using Side-by-Side Bar Graphs

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Solution Overview

Problem

Current methods for endotoxin testing, particularly those using Limulus amebocyte lysate (LAL), are cumbersome, prone to contamination, and lack efficient data presentation, making them time-consuming and costly.

Innovation Solution

The development of a system and method for presenting endotoxin concentration measurements in a graphical user interface (GUI) or report, utilizing a processor to display side-by-side graphs with vertically oriented bars, allowing for simultaneous comparison of endotoxin reactions across multiple wells.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional LAL testing methods are used, then endotoxin detection can be performed, but the process is time-consuming and costly due to manual operations and lack of efficient data presentation

Engineering Contradiction:
Improvetesting efficiencyVSAvoidtime for data presentation
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent replaces manual mechanical data presentation with an automated computer-based graphical user interface system. The processor automatically generates and displays visual representations of endotoxin concentrations, eliminating the need for manual data compilation and presentation, thereby significantly improving testing efficiency and reducing time loss.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If multiple wells are tested simultaneously, then productivity increases, but data interpretation becomes more complex without proper visualization

Engineering Contradiction:
Improvethroughput of multiple wellsVSAvoiddata interpretation
Core Design Contradiction:
ProductivityVSEase of operation

Solution Approach 1:

The patent employs color-coded visual elements in the graphical user interface to represent different endotoxin concentration levels and test results across multiple wells. This color-based visualization system enables operators to quickly interpret data from numerous wells simultaneously, maintaining high throughput while significantly improving ease of operation and data interpretation.

Inventive Principle:
Principle #32Color changes

3Ease of operation

If manual testing procedures are used, then flexibility is maintained, but contamination risk increases

Engineering Contradiction:
Improveflexibility in testingVSAvoidcontamination risk
Core Design Contradiction:
Ease of operationVSObject-affected harmful factors

Solution Approach 1:

The patent implements automated data processing and presentation systems that reduce the need for manual intervention in the testing process. The system automatically collects, processes, and visualizes data from multiple wells, maintaining operational flexibility while minimizing human contact with samples and reagents, thereby reducing contamination risk.

Inventive Principle:
Principle #25Self-service

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach simplifies the visualization of endotoxin testing progress, reduces the risk of contamination, and enhances data interpretation, thereby streamlining the testing process and reducing costs.

Implementation Method 1

Clotting will occur when the hemocyte lysate is exposed to the endotoxin. Hemocyte lysate is amebocyte lysate produced from the hemolymph of various horseshoe crab species

Methodology Applied
Scientific EffectLAL reaction:

Data Source

PatentEP3935393B1Method and system for visualization of endotoxins in a fluid sample
Publication Date: 2025.05.07 BL TECHNOLOGY INC
  • EP3935393B1 patent drawingFigure 1
  • EP3935393B1 patent drawingFigure 2A
  • EP3935393B1 patent drawingFigure 2B

AI summary

Exemplified methods and systems facilitate presentation of data derived from measurements of endotoxins in fluid samples. In particular, the exemplified methods and systems facilitate presentation of such measurements in a graphical user interface and/or in a report for endotoxin concentrations in a fluid sample. The presentation facilitates a unified and intuitive graphic visualization that are presented within a single interactive interface and/or report.