Engineered Amylase Sequences for Heat-Stable Detergent Cleaning
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current amylase enzymes lose activity at high temperatures and in harsh detergent formulations, impacting their effectiveness in cleaning applications.
Innovation Solution
Genetically engineered amylases with specific amino acid sequences, offering at least 94% to 100% identity to SEQ ID NOs, retain activity and performance under high temperatures and in detergent formulations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional amylase enzymes are used in cleaning compositions, then they can remove starch stains under normal conditions, but they lose activity at high temperatures and in harsh detergent formulations
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of amylase enzymes through genetic engineering. Specific substitutions include replacing hydrophobic residues with hydrophilic ones at positions 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 to enhance thermostability and formulation compatibility while maintaining catalytic activity
2Adaptability or versatility
If conventional amylase enzymes are used in detergent formulations, then they can function in cleaning products, but they do not retain activity in harsh detergent formulations
Solution Approach 1:
The patent modifies enzyme parameters by substituting amino acid residues to change the surface properties and charge distribution of the amylase molecule. This enables the enzyme to tolerate harsh detergent conditions including high alkalinity, presence of surfactants, and other formulation components while maintaining catalytic function
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The engineered amylases maintain effective starch stain removal performance in cleaning compositions, even at elevated temperatures and in challenging formulations.
Implementation Method 1
Amylase enzymes have been employed in the removal of starch stains
Implementation Method 2
Amylases have been employed in the removal of starch stains
Data Source
AI summary
Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. Fragments of parent amylase enzymes activity and methods of using the fragments for making genetically engineered enzymes having amylase activity. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.