Genetically Engineered Microorganisms for Homogeneous Biofuel Production

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Solution Overview

Problem

Current methods for producing biodiesel involve transesterification of triacylglycerides, resulting in heterogeneous products and economic inefficiencies due to the production of unwanted side products like glycerin, and there is a need for more efficient and cost-effective methods to produce biofuels from renewable carbon sources.

Innovation Solution

Genetically engineered microorganisms are developed to produce fatty acid derivatives, such as short chain alcohols, fatty alcohols, fatty acid esters, and hydrocarbons, using renewable carbon sources, which can be used directly as biofuels without the need for special storage or transportation methods, and these microorganisms are engineered to produce homogeneous products with defined carbon chain lengths and saturation levels.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If transesterification of triacylglycerides is used to produce biodiesel, then fatty acid esters are produced, but heterogeneous products and unwanted side products (glycerin) are generated causing economic inefficiencies

Engineering Contradiction:
Improvebiodiesel production efficiencyVSAvoidwaste product (glycerin) generation
Core Design Contradiction:
ProductivityVSLoss of substance

Solution Approach 1:

The patent extracts and eliminates the problematic byproduct generation step by using genetically engineered microorganisms that directly produce fatty acid derivatives through metabolic pathways, bypassing the transesterification process that generates glycerin waste

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention changes the fundamental production parameter from chemical transesterification to biological fermentation, transforming the production mechanism to eliminate heterogeneous product formation and unwanted side products while maintaining biodiesel production capability

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If genetically engineered microorganisms are used to produce fatty acid derivatives, then homogeneous products with defined carbon chain lengths are produced, but the device complexity increases due to genetic engineering requirements

Engineering Contradiction:
Improveproduct homogeneity and carbon chain length definitionVSAvoidgenetic engineering process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The genetically engineered microorganisms serve themselves by using their own metabolic machinery to produce homogeneous fatty acid derivatives with defined carbon chain lengths, eliminating the need for complex external separation and purification equipment

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The invention uses dynamic genetic engineering approaches where microorganism strains can be adapted and optimized for specific product specifications, allowing flexible adjustment of carbon chain lengths and product properties through genetic modification rather than fixed mechanical processes

Inventive Principle:
Principle #15Dynamics

3Productivity

If current transesterification methods are used, then biodiesel is produced, but separation and storage of side products is required increasing process complexity and cost

Engineering Contradiction:
Improvebiodiesel production rateVSAvoidseparation and storage system complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The invention extracts and removes the separation and storage requirements by engineering microorganisms to produce only the desired fatty acid derivative products without generating significant unwanted byproducts that would require complex separation and storage infrastructure

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

Instead of producing heterogeneous mixtures and then separating them, the invention inverts the approach by directly producing homogeneous products through engineered metabolic pathways, eliminating the need for downstream separation operations

Inventive Principle:
Principle #13The other way round (Inversion)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The approach allows for the production of biofuels and specialty chemicals with reduced production costs and increased efficiency by eliminating the need for separation and storage of side products, resulting in a more cost-effective and environmentally friendly biofuel production process.

Implementation Method 1

The modified microorganisms can then be used in a fermentation process to produce useful fatty acid derivatives using the renewable carbon source (biomass) as a starting material

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentEP2840131B1Production of fatty acids and derivatives thereof
Publication Date: 2019.10.02 GENOMATICA INC
  • EP2840131B1 patent drawingFigure 1
  • EP2840131B1 patent drawingFigure 2
  • EP2840131B1 patent drawingFigure 3

AI summary

Genetically engineered microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use.