Engineered Mitochondria via Exogenous Membrane Coating
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Solution Overview
Problem
Biologically active free mitochondria extracted from cells are unstable and lack targeted effects, resulting in unsatisfactory therapeutic outcomes for mitochondrial dysfunction-related diseases.
Innovation Solution
Engineered mitochondria are produced by attaching exogenous cell membranes to the outer membranes of exogenous mitochondria, providing a membrane-like environment that stabilizes their biological activity and enhances therapeutic effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If biologically active free mitochondria are extracted from cells or tissue, then they can be administered to treat mitochondrial dysfunction, but they are extremely unstable and lose biological activity quickly
Solution Approach 1:
The patent creates a composite structure by coating exogenous cell membranes onto the outer membranes of exogenous mitochondria. This composite mitochondrial structure (comprising the mitochondrial inner membrane, outer membrane, and coated cell membrane) enhances the stability and prolongs the biological activity duration of the mitochondria when administered for treatment
2Reliability
If biologically active free mitochondria are extracted from cells or tissue, then they can be administered to treat mitochondrial dysfunction, but they lack targeted effects on affected tissue
Solution Approach 1:
The patent applies local quality modification by selectively coating the outer membrane of mitochondria with cell membranes. The cell membrane coating provides localized targeting capabilities while the mitochondrial structures maintain their core functions. Different cell membrane types can be used to target different affected tissues, enhancing the adaptability and targeted delivery of the therapeutic mitochondria
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The engineered mitochondria exhibit higher biological activity and improved therapeutic effects on mitochondrial dysfunction-related disorders, with increased ATP production and reduced ROS release, leading to better tissue targeting and disease treatment.
Implementation Method 1
Engineered mitochondria are formed by attaching exogenous cell membranes to outer membranes of exogenous mitochondria
Implementation Method 2
the mixture is subjected to 2-5 minutes of ultrasonication in a water bath at 4° C.
Implementation Method 3
followed by centrifugation at 3500 g for 10-15 minutes
Data Source
AI summary
The invention provides engineered mitochondria and a preparation method thereof, and relates to the technical field of mitochondria. The engineered mitochondria are formed by attaching exogenous cell membranes to outer membranes of exogenous mitochondria. The preparation method comprises the following steps: S1: extracting exogenous cell membranes from cells; S2: separating and extracting exogenous mitochondria from cells or tissue; and S3: mixing the separated and extracted exogenous mitochondria with the exogenous cell membranes in a specific ratio, thereby attaching the exogenous cell membranes to the outer membranes of the exogenous mitochondria to obtain the engineered mitochondria. The invention enables the production of engineered mitochondria with enhanced biological activity, exhibiting improved therapeutic effects on mitochondrial dysfunction-related disorders.