Entada Seed Extract Enrichment via Enzymatic Hydrolysis
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for obtaining extracts from Entada seeds are limited by the presence of metabolites in precursor forms that are less biologically active, and the instability of entadamide A in water, leading to unwanted by-products and loss of biological activity, which is not suitable for cosmetic applications.
Innovation Solution
A process involving sequential enzymatic hydrolysis with controlled conditions, including activation and deactivation of endogenous β-glucosidases, to selectively convert β-D-glucopyranoside forms of entadamide A into its aglycone form, avoiding concomitant hydrolysis of other constituents and using organic solvents to inhibit enzymatic activity, resulting in a stable extract enriched in entadamide A.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional extraction methods are used to obtain Entada seed extract, then the extraction process is simple, but the metabolites remain in precursor forms (β-D-glucopyranoside forms) that are less biologically active
Solution Approach 1:
The patent applies preliminary action by activating endogenous β-glucosidases before extraction to pre-convert precursor metabolites into their biologically active aglycone forms. This preliminary enzymatic activation ensures that the metabolites are in the desired active form before the extraction process begins, resolving the contradiction between maintaining simple extraction and achieving high biological activity.
Solution Approach 2:
The patent replaces mechanical/chemical extraction methods with an enzymatic system using endogenous β-glucosidases. This substitution allows for selective conversion of specific metabolite precursors into active forms while maintaining relatively simple process conditions, thus improving biological activity without proportionally increasing complexity.
2Ease of manufacture
If entadamide A is extracted in water, then the extraction is straightforward, but entadamide A becomes unstable and forms unwanted by-products
Solution Approach 1:
The patent changes the physical-chemical parameters of the extraction medium by using hydro-alcoholic solvents (combining water with organic alcohols). This parameter modification maintains extraction simplicity while significantly improving entadamide A stability and preventing by-product formation, thus resolving the contradiction between ease of manufacture and composition stability.
3Reliability
If enzymatic hydrolysis is performed to convert β-D-glucopyranoside forms to aglycone, then biological activity increases, but other constituents may be concomitantly hydrolyzed
Solution Approach 1:
The patent employs self-service by utilizing endogenous β-glucosidases naturally present in the Entada seeds themselves rather than adding exogenous enzymes. These endogenous enzymes selectively hydrolyze the β-D-glucopyranoside forms of metabolites while leaving other constituents intact, achieving both high conversion efficiency and excellent selectivity without requiring complex enzyme selection or addition procedures.
Solution Approach 2:
The patent selectively extracts and utilizes only the specific endogenous β-glucosidase activity needed for converting metabolite precursors, while leaving other enzymatic activities in the system. This selective extraction of functional activity enables precise control over which constituents are hydrolyzed, maintaining manufacturing precision while achieving high metabolite conversion efficiency.
4Stability of the object's composition
If the extract contains entadamide A in glycosylated form, then the extract is stable in solution, but the biological activity is reduced
Solution Approach 1:
The patent applies preliminary action by performing enzymatic hydrolysis during the extraction process itself to convert glycosylated forms to aglycones before the extract is finalized. This preliminary conversion ensures that the final extract contains the biologically active aglycone forms while maintaining stability through the use of hydro-alcoholic solvents, thus resolving the contradiction between stability and biological activity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process achieves a high conversion rate of entadamide A glucone to aglycone, maintaining biological activity and stability, producing an extract suitable for cosmetic use without the need for separation or purification steps, with enhanced photoprotective and antioxidant properties.
Implementation Method 1
a phase of enzymatic hydrolysis of the dispersion obtained in step (i) by heat treatment at a temperature between about 25 and about 100° C. and for a time period of between about 2 minutes and about 12 hours
Implementation Method 2
activation and deactivation of endogenous β-glucosidases, to selectively convert β-D-glucopyranoside forms of entadamide A into its aglycone form
Implementation Method 3
activation and deactivation of endogenous β-glucosidases, to selectively convert β-D-glucopyranoside forms of entadamide A into its aglycone form
Implementation Method 4
using organic solvents to inhibit enzymatic activity, resulting in a stable extract enriched in entadamide A
Data Source
AI summary
The invention relates to a method for producing an extract of seeds of the Entada genus by selective hydrolysis of the metabolites accumulated in the seeds. The invention also relates to the extract produced using such a method, to a composition comprising such an extract, and to the cosmetic and dermocosmetic applications of such an extract.