Enzymatic Solubilization of Cereal Bran for Food Applications

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Solution Overview

Problem

There is a need to improve the utilization of cereal bran in food products without affecting the appearance, structure, or taste, and to increase the nutritional and health benefits of existing cereal products, while reducing the amount of bran that goes to low-value applications like cattle feed.

Innovation Solution

A method involving the sequential treatment of cereal bran with xylanase, cellulase, and starch-modifying enzymes like alpha-amylase, pullulanase, and transglucosidase to enhance solubilization, maintaining starch and producing higher yields of arabinoxylan oligosaccharides and soluble material, which can be used in food products without significant impact on texture or taste.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If cereal bran is solubilised using traditional methods, then the nutritional value is improved, but the product appearance, structure and taste are significantly affected

Engineering Contradiction:
Improvesoluble fraction yieldVSAvoidproduct structure
Core Design Contradiction:
Quantity of substanceVSShape

Solution Approach 1:

The patent applies parameter changes by carefully controlling enzymatic treatment conditions (enzyme types, concentrations, treatment time, temperature, pH) to achieve partial solubilisation that releases nutritional compounds while preserving the structural integrity of the bran matrix. This selective parameter optimization allows obtaining soluble fractions without completely disrupting the product structure.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent employs partial action by using controlled enzymatic hydrolysis that does not completely degrade the bran structure. Instead of full solubilisation, the treatment is optimized to release specific nutritional compounds (arabinoxylan, phenolic compounds, vitamins) while leaving sufficient structural components intact to maintain product appearance and texture.

Inventive Principle:
Principle #16Partial or excessive action

2Quantity of substance

If more cereal bran is used in food products, then the health benefits are increased, but the taste and appearance are deteriorated

Engineering Contradiction:
Improvebran content in productVSAvoidnegative sensory properties
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The patent extracts beneficial compounds (arabinoxylan oligosaccharides, phenolic compounds, vitamins, minerals) from the bran matrix through enzymatic solubilisation. These extracted compounds can then be added back to products in controlled amounts, providing health benefits without the negative sensory properties associated with high bran content.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses enzymes as intermediaries to transform insoluble bran components into soluble forms. This enzymatic mediation converts the harsh, fibrous bran structure into gentle, soluble compounds that can enhance product nutrition without compromising sensory quality. The enzymes act as mediators between the raw bran material and the final product requirements.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Quantity of substance

If enzymatic treatment is applied to cereal bran, then the nutritional value is enhanced, but the processing complexity increases

Engineering Contradiction:
Improvenutritional compounds releasedVSAvoidprocessing steps
Core Design Contradiction:
Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent combines multiple enzymatic treatments into a single integrated process step. Instead of separate treatments for different nutritional compounds, the method uses a cocktail of enzymes (xylanases, arabinases, feruloyl esterases, etc.) that work simultaneously to release multiple nutritional compounds in one operation, simplifying the overall processing complexity.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The patent employs multi-functional enzymes that can perform multiple roles. For example, certain enzymes can simultaneously hydrolyze different bond types or work across different pH and temperature ranges, reducing the number of separate processing steps needed and simplifying the overall treatment protocol.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method allows for the effective solubilization of cereal bran, increasing the utilization of cereals, enhancing the nutritional value of products, and reducing the need for low-value applications, while maintaining product quality and health benefits.

Implementation Method 1

treating said particulate cereal bran in liquid suspension sequentially in any order without the removal of any components or simultaneously with: a xylanase and a cellulase; one or more starch modifying enzyme(s) selected from the group consisting of an alpha-amylase, a pullulanase, isoamylase and a beta-amylase; and a transglucosidase having an activity defined as EC 2.4.1.24

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Hydrolysis

Data Source

PatentEP2387327B1Enzymatic generation of oligasaccharides from cereals or cereal bi-streams
Publication Date: 2018.03.21 INT N&H DENMARK APS
  • EP2387327B1 patent drawingFigure 1
  • EP2387327B1 patent drawingFigure 2
  • EP2387327B1 patent drawingFigure 3

AI summary

The present invention relates to the solubilisation of cereal bran, for preparing compositions comprising soluble fractions of cereal bran and the use of these compositions comprising solubilised cereal bran for the preparation of food products, such as bread.