Enzymatic Curing Agent Production via Fluid Phase Separation
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Solution Overview
Problem
Existing methods for using natural antimicrobial enzyme systems, such as the lactoperoxidase system, face issues with excessive enzyme consumption, low reaction yields, and rapid breakdown of oxygenated chemical species, particularly when enzymes are packaged in dry form or immobilized, leading to inefficient decontamination and purification processes.
Innovation Solution
A process for enzymically producing a treatment agent in a fluid state, comprising a peroxidase enzyme, an oxidizable substrate, and an oxygen donor, which generates stable oxygenated chemical species by forming an aqueous reaction bath and separating it into fractions to obtain a treatment agent, using aggregating agents like floccules or coagulates to maintain the enzyme in a free state and recycle it.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If enzymes are packaged in dry form or immobilized, then they can be stored and reused, but enzyme consumption increases and reaction yields decrease
Solution Approach 1:
The invention changes the physical state of the enzyme from solid/dry form to fluid state, allowing the enzyme to remain in solution while being continuously supplied to the reaction system. This parameter change resolves the contradiction by maintaining enzyme reusability through continuous circulation while preserving high reaction yields through optimal enzyme-substrate contact in fluid phase.
Solution Approach 2:
The invention implements a dynamic system where the enzyme solution is continuously circulated between the reaction chamber and storage/processing units. This dynamic approach allows the enzyme to be reused indefinitely while maintaining high catalytic activity, resolving the contradiction between reusability and reaction yield by enabling both simultaneously through continuous motion and circulation.
2Ease of manufacture
If enzymes are packaged in dry form, then storage is simplified, but enzyme consumption increases proportionally to treatment area and volume
Solution Approach 1:
The invention creates a self-sustaining system where the enzyme solution is continuously recycled and reused within the system. The enzyme performs catalytic cycles repeatedly without being consumed, and the fluid state allows automatic circulation and distribution to multiple treatment areas, eliminating the need for repeated enzyme additions and reducing overall enzyme consumption while maintaining storage simplicity through centralized enzyme solution reservoirs.
3Duration of action of stationary object
If immobilized enzymes are used, then repeated use is enabled, but oxygenated chemical species break down rapidly
Solution Approach 1:
The invention implements continuous circulation of the enzyme solution through the reaction system, enabling the enzyme to be reused repeatedly while maintaining optimal conditions for oxygenated chemical species stability. The dynamic fluid state allows controlled residence time and continuous replenishment, resolving the contradiction by enabling both long-term enzyme reuse and short-term species stability through continuous motion and circulation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This process allows for the production of a stable treatment agent with high yield and endurance, capable of maintaining oxygenated chemical species for extended periods, effectively addressing the limitations of previous methods by enabling continuous and efficient generation of bacteriostatic and bactericidal solutions for decontamination and purification.
Implementation Method 1
an agent of enzymic catalysis, comprising at least one enzyme of the peroxidase type
Implementation Method 2
the lactoperoxidase catalyses the thiocyanate oxidation reaction
Implementation Method 3
an oxygen donor: hydrogen peroxide
Implementation Method 4
using aggregating agents like floccules or coagulates
Implementation Method 5
using aggregating agents like floccules or coagulates
Data Source
AI summary
The invention relates to a method for the enzymatic production of a curing agent in its fluid state, e.g. liquid, comprising, in free phase, at least one oxygenated chemical species. Said method consists in bringing into contact at least one enzymatic catalysts agent, comprising at lease one peroxidase-type enzyme; an oxidizable substrate in aqueous phase that can be oxidized by the action of an oxygen donor, by catalysis by said enzymatic catalysis agent, generating said oxygenated chemical species in free phase; and said oxygen donor. The inventive method is characterized in that: e) an aqueous reaction bath is formed comprising, in addition to the oxidizable substrate and the oxygen donor, said enzymatic catalysis agent in divided solid phase, but in free phase, distributed is said bath, which may be set in motion; f) the aqueous reaction bath is separated into a fraction enriched with the enzymatic catalysis agent in divided solid phase and a fraction free from said catalysis agent, from which the curing agent is obtained.