Enzymatic Peracid Formulation With Low-LogP Cosolvent Stability
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Solution Overview
Problem
Multi-component enzymatic peroxycarboxylic acid generation systems face issues with storage stability and mixing efficiency due to insoluble carboxylic acid ester substrates, which affect the production and delivery of peroxycarboxylic acid, and the use of organic cosolvents can be detrimental to enzyme activity.
Innovation Solution
A multi-component formulation comprising a CE-7 enzyme catalyst with specific amino acid motifs, a carboxylic acid ester substrate, and a cosolvent with a log P value of less than 2, combined with hydrogen peroxide and a stabilizer, to enhance storage stability and mixing characteristics, allowing for efficient production of peroxycarboxylic acid.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If carboxylic acid ester substrates are used in multi-component enzymatic peroxycarboxylic acid generation systems, then peroxycarboxylic acid production is enabled, but storage stability and mixing efficiency deteriorate due to substrate insolubility
Solution Approach 1:
The carboxylic acid ester substrate is pre-dissolved in a cosolvent to create a stable, homogeneous solution before use. This preliminary dissolution action prevents insolubility issues during storage and mixing, allowing the substrate to remain stable in the multi-component system while maintaining readiness for peroxycarboxylic acid production
Solution Approach 2:
A cosolvent is introduced as an intermediary substance between the carboxylic acid ester substrate and water. This cosolvent mediates the solubility issue by dissolving the hydrophobic ester substrate, enabling it to mix properly with aqueous components while maintaining substrate availability for enzymatic conversion to peroxycarboxylic acid
2Productivity
If carboxylic acid ester substrates are used, then peroxycarboxylic acid can be produced, but mixing efficiency deteriorates due to high viscosity from insoluble substrates
Solution Approach 1:
The substrate is pre-dissolved in the cosolvent to create a homogeneous solution before mixing with other components. This preliminary dissolution eliminates viscosity problems that would otherwise occur during mixing, ensuring easy and efficient mixing of all components without aggregation or phase separation
Solution Approach 2:
The cosolvent acts as a mediator that reduces the viscosity and improves the flow characteristics of the substrate-containing component. By dissolving the carboxylic acid ester in the cosolvent, the mixture becomes more fluid and easier to mix with aqueous hydrogen peroxide and enzyme solutions, thereby improving overall mixing efficiency
3Ease of operation
If organic cosolvents are used to improve substrate solubility, then mixing efficiency improves, but enzyme activity deteriorates
Solution Approach 1:
The log P value parameter of the cosolvent is specifically controlled to be less than 2. This parameter change identifies cosolvents with appropriate polarity that can dissolve carboxylic acid esters without being too hydrophobic to harm enzyme activity. By selecting cosolvents within this specific parameter range, both mixing efficiency and enzyme reliability are optimized
Solution Approach 2:
The cosolvent creates a local microenvironment around the enzyme and substrate that facilitates solubility and mixing while maintaining conditions suitable for enzyme activity. The cosolvent concentrates in regions where substrate dissolution is needed, allowing the enzyme to function in a mostly aqueous environment where it remains stable and active
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The formulation improves storage stability and mixing efficiency, enabling the rapid production of efficacious peroxycarboxylic acid concentrations suitable for disinfection, bleaching, and sanitization applications without significant loss of enzyme activity.
Implementation Method 1
enzymes structurally classified as members of the CE-7 family of carbohydrate esterases (i.e., cephalosporin C deacetylases [CAHs] and acetyl xylan esterases [AXEs]) that are characterized by significant perhydrolysis activity for converting carboxylic acid esters (in the presence of a suitable source of peroxygen, such as hydrogen peroxide) into peroxycarboxylic acids
Implementation Method 2
A multi-component formulation comprising a CE-7 enzyme catalyst with specific amino acid motifs, a carboxylic acid ester substrate, and a cosolvent with a log P value of less than 2, combined with hydrogen peroxide and a stabilizer, to enhance storage stability and mixing characteristics
Implementation Method 3
Peroxycarboxylic acids can be prepared by the chemical reaction of a carboxylic acid and hydrogen peroxide
Implementation Method 4
A multi-component formulation comprising a CE-7 enzyme catalyst with specific amino acid motifs, a carboxylic acid ester substrate, and a cosolvent with a log P value of less than 2, combined with hydrogen peroxide and a stabilizer, to enhance storage stability
Data Source
Figure 1a
Figure 1b
Figure 2a~2b
AI summary
Disclosed herein are multi-component formulations for enzymatically producing aqueous solutions of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid formulations comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity in powder form, an excipient, a buffer and a cosolvent, and hydrogen peroxide.