Enzymatic Straw Delignification for High-Strength Cellulose Fibres

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Solution Overview

Problem

Current pulping processes, such as mechanical and chemical pulping, generate significant waste and fail to effectively utilize by-products like lignin and hemicellulose, resulting in lower quality products and environmental concerns.

Innovation Solution

A method involving dry mechanical treatment, sieving, and mild enzymatic delignification of straw to produce a cellulose-rich fibre fraction while separating and preserving lignin, hemicellulose, and oligosaccharide-rich by-products, which can be further processed for commercial use.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If mechanical pulping is used, then pulp yield and cost are improved, but fibre strength and product quality deteriorate due to high lignin content

Engineering Contradiction:
Improvepulp yieldVSAvoidfibre strength
Core Design Contradiction:
ProductivityVSStrength

Solution Approach 1:

The invention extracts and removes lignin from the straw material through enzymatic delignification, separating it from the cellulose fibers. This allows the cellulose-rich fiber fraction to be obtained with improved strength properties while the lignin is recovered as a valuable by-product, resolving the contradiction between maintaining high pulp yield and improving fiber strength.

Inventive Principle:
Principle #2Taking out (Extraction)

2Strength

If chemical pulping (Kraft process) is used, then fibre strength is improved through lignin removal, but waste generation and environmental harm increase

Engineering Contradiction:
Improvefibre strengthVSAvoidwaste generation
Core Design Contradiction:
StrengthVSObject-generated harmful factors

Solution Approach 1:

The invention changes the parameters of the delignification process by using mild enzymatic treatment instead of harsh chemical conditions. The process operates at lower temperatures (50-70°C), neutral to slightly alkaline pH (7-9), and uses enzymes rather than strong chemicals, thereby achieving effective lignin removal for improved fiber strength while minimizing waste generation and environmental harm.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

Instead of discarding lignin as waste in conventional chemical pulping, the invention recovers it as a valuable by-product. The delignified liquid fraction containing lignin, hemicellulose, and oligosaccharides is separated and can be utilized for energy production, chemical synthesis, or other industrial applications, thus reducing waste generation while maintaining fiber strength improvement.

Inventive Principle:
Principle #34Discarding and recovering

3Strength

If chemical pulping (sulfite process) is used, then delignification is achieved, but pollution and chemical recovery limitations occur

Engineering Contradiction:
Improvedelignification efficiencyVSAvoidpollution
Core Design Contradiction:
StrengthVSObject-affected harmful factors

Solution Approach 1:

The invention replaces the mechanical/chemical pulping system with a biological system using enzymes. Instead of using sulfurous acid and sulfite/bisulfite salts that cause pollution and chemical recovery issues, the process employs cellulases, hemicellulases, and lignin peroxidases to achieve delignification under mild conditions, eliminating pollution problems while maintaining effective lignin removal.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach reduces waste generation, retains fibre strength, and allows for the isolation of valuable by-products, enhancing the pulping process's environmental and commercial viability.

Implementation Method 1

a mild enzymatic delignification to obtain a cellulose rich fibre fraction

Methodology Applied
Scientific EffectEnzymatic delignification: Enzyme

Implementation Method 2

The combination of sulfurous acid and sulfite/bisulfite salts degrade and dissolves the lignin in the wood known as delignification

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 3

Mechanical pulping results in little removal of the lignin content in the organic material

Methodology Applied
Scientific EffectMechanical breakdown: Mechanical Force

Implementation Method 4

The process ultilize strong alkaline conditions with pH in the range 12-14, high cooking temperatures around 170°C, long reaction times and pressurized vessels in the delignification of the wood

Methodology Applied
Scientific EffectAlkaline dissolution: Solvation

Implementation Method 5

The combination of sulfurous acid and sulfite/bisulfite salts degrade and dissolves the lignin in the wood known as delignification. This process rely mainly on acidic cleavage of ether bonds present in lignin

Methodology Applied
Scientific EffectAcidic cleavage: Hydrolysis

Implementation Method 6

The process ultilize strong alkaline conditions with pH in the range 12-14, high cooking temperatures around 170°C, long reaction times and pressurized vessels in the delignification of the wood. These harsh reaction conditions results in cleavage of the ether bonds by nucleophilic sulfide and bisulfides present in the mixture

Methodology Applied
Scientific EffectNucleophilic attack: Chemical Bonding

Data Source

PatentEP3538592B1Method of preparing a cellulose rich fibre fraction and valuable by-products
Publication Date: 2023.01.04 TEKNOLOGISK INSTITUT
  • EP3538592B1 patent drawingFigure 1
  • EP3538592B1 patent drawingFigure 2
  • EP3538592B1 patent drawingFigure 3

AI summary

The present invention relates to a method for preparing a cellulose rich fibre fraction, said method comprising the steps of: (i) providing straw, (ii) subjecting the straw to a dry mechanical treatment, (iii) subjecting the material obtained in (ii) to a sieving treatment and obtaining at least two fractions, the first fraction passing through the sieve mesh and the second fraction being retained by the sieve mesh, (iv) suspending the second fraction in an aqueous solution comprising one or more enzymes, adjusting pH of the mixture in the range of 4-6.5 and adjusting temperature of the mixture in the range of 30-70°C, (v) subjecting the mixture obtained in step (iv) to a continuous or intermittent mechanical defibration under agitation, (vi) separating the material obtained in step (v) into a cellulose rich fibre fraction and a liquid fraction comprising suspended and/or dissolved lignin, hemicellulose and oligosaccharides, (vii) subjecting the liquid fraction obtained in (vi) to lignin precipitation and obtaining a precipitated lignin rich fraction and a hemicellulose and oligosaccharide rich fraction, and (viii) separating the precipitated lignin rich fraction obtained in step (vii) from the hemicellulose and oligosaccharide rich fraction.