Enzymatic Tributyrin-Cyclodextrin Inclusion Complex
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Solution Overview
Problem
Tributyrin, a potential substitute for antibiotic feed additives, faces challenges such as easy dissociation, volatility, and unpleasant smell, and existing methods for forming inclusion complexes with cyclodextrin are costly and inefficient, with high oil content and poor solubility.
Innovation Solution
An enzymatic preparation method combining the synthesis of cyclodextrin and inclusion of tributyrin using cyclodextrin glucosyltransferase (CGT) enzyme, where tributyrin is added during the enzymatic synthesis, followed by emulsification and spray drying, reducing costs and improving solubility and dispersibility.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If cyclodextrin is directly used as wall material for tributyrin inclusion, then the peculiar smell and poor fluidity of tributyrin are improved, but the cost increases and solubility decreases
Solution Approach 1:
The patent combines the cyclodextrin synthesis process with the tributyrin inclusion process into a single integrated system. The cyclodextrin glucosyltransferase enzyme simultaneously performs cyclization of starch to form cyclodextrin and facilitates inclusion of tributyrin within the cyclodextrin cavity, eliminating the need for separate purification and inclusion steps, thereby reducing cost while maintaining improved solubility and smell masking
Solution Approach 2:
The cyclodextrin acts as an intermediary substance that mediates between the hydrophobic tributyrin and the aqueous environment. The cyclodextrin cavity encapsulates tributyrin, providing solubility enhancement and smell masking, while the integrated enzymatic process ensures cost-effective production
2Reliability
If traditional separate processes of cyclodextrin production and tributyrin inclusion are used, then the inclusion complex can be formed, but the production cost increases and efficiency decreases
Solution Approach 1:
The patent merges two separate industrial processes (cyclodextrin production and tributyrin inclusion) into a single integrated enzymatic reaction system. The CGTase enzyme catalyzes both the cyclization of starch to form cyclodextrin and the subsequent inclusion of tributyrin, achieving reliable complex formation while significantly improving productivity and reducing production costs
Solution Approach 2:
The integrated process enables continuous useful action where the cyclodextrin is synthesized and immediately utilized for tributyrin inclusion without interruption or separation. This continuous process eliminates idle time and intermediate handling, thereby improving overall production efficiency and reducing costs
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in a stable and cost-effective inclusion complex with improved solubility and shelf life, reducing the unpleasant smell and oil content, making it suitable as a substitute for antibiotic additives in animal feed.
Implementation Method 1
An enzyme is added into the starch milk or maltodextrin solution for liquefaction reaction and cyclization reaction
Implementation Method 2
cyclodextrin is formed by connecting 6 or more D-glucopyranoses with α-1,4-glycosidic bonds through a cyclization reaction
Implementation Method 3
cyclodextrin can form inclusion complexes with many hydrophobic guest molecules due to its external hydrophilicity and internal hydrophobicity
Implementation Method 4
after reaction, an emulsifier is added, and homogenization and spray drying are carried out
Implementation Method 5
after reaction, an emulsifier is added, and homogenization and spray drying are carried out
Data Source
AI summary
The disclosure relates to an enzymatic preparation method of inclusion complexes of tributyrin, and belongs to the technical field of oil microencapsulation. The disclosure combines enzymatic synthesis of cyclodextrin and inclusion of tributyrin with cyclodextrin, including enzymatic preparation of cyclodextrin with a CGT enzyme. Tributyrin is added in the preparation process; after reaction, Tween is added, and homogenization and spray drying are carried out. The effect of the finally obtained tributyrin powder is much better than that of single inclusion of tributyrin with cyclodextrin. The disclosure is simple in process, low in cost and convenient in operation; reaction processes are free of toxicity and pollution; there are no toxic reagent residues; the inclusion effect is obvious, and better utilization of a nutritional additive tributyrin in actual production is facilitated.