Enzyme-Producing Bacteria for Aggregate Cementation

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Solution Overview

Problem

The construction industry relies heavily on high-embodied energy materials like concrete and steel, which contribute significantly to carbon dioxide emissions, and traditional clay brick manufacturing also has a substantial carbon footprint due to its reliance on non-renewable resources and energy-intensive processes.

Innovation Solution

The method involves using enzyme-producing bacteria like Sporosarcina Pasteurii to induce calcite precipitation in loose aggregate, such as sand, to form a solid construction material through microbial-induced calcite precipitation (MICP), reducing the need for high-energy materials and minimizing carbon emissions by creating a locally sourced, biologically grown building material.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Object-generated harmful factors

If traditional clay brick manufacturing is used, then construction material is produced, but carbon dioxide emissions are high due to coal-powered kilns

Engineering Contradiction:
Improvecarbon dioxide emissionsVSAvoidmanufacturing process simplicity
Core Design Contradiction:
Object-generated harmful factorsVSEase of manufacture

Solution Approach 1:

The patent replaces the thermal/chemical system of coal-powered kilns with a biological system using urease-producing bacteria. The bacteria catalyze the decomposition of urea to produce ammonia and carbon dioxide in situ, which then react with calcium ions to form calcite that cements the aggregate. This biological substitution eliminates the need for high-temperature firing and external CO2 emissions.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The bacteria are applied to the loose aggregate and autonomously perform the cementation function. The urease-producing bacteria self-catalyze the chemical reactions needed to form calcite bonds between sand grains, eliminating the need for external energy input or complex manufacturing equipment. The system serves itself by using the bacteria's natural enzymatic activity to achieve the binding function.

Inventive Principle:
Principle #25Self-service

2Productivity

If concrete masonry units with Portland cement are used, then manufacturing speed is improved, but carbon dioxide emissions from cement production increase

Engineering Contradiction:
Improvemanufacturing speedVSAvoidcarbon dioxide emissions from cement production
Core Design Contradiction:
ProductivityVSObject-generated harmful factors

Solution Approach 1:

The patent replaces the industrial chemical process of Portland cement production with a biological process using urease-producing bacteria. Instead of heating limestone to high temperatures to produce cement, the bacteria catalyze urea decomposition and subsequent calcite precipitation at ambient conditions, achieving similar binding results without the high emissions associated with cement manufacturing.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the fundamental parameters of the cementation process by operating at ambient temperature and pressure rather than the high temperatures required for Portland cement production. The biochemical pathway through urease catalysis allows the same functional outcome (aggregate bonding) to be achieved under vastly different, more environmentally friendly conditions.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If fired clay bricks are manufactured, then structural material is produced, but the process takes 3-20 days depending on equipment

Engineering Contradiction:
Improvemanufacturing timeVSAvoidenergy consumption
Core Design Contradiction:
ProductivityVSUse of energy by stationary object

Solution Approach 1:

The patent replaces the thermal processing system of fired brick manufacturing with a biochemical system. The urease-producing bacteria catalyze rapid calcite formation at ambient temperatures, achieving structural bonding in a fraction of the time required for traditional firing and cooling cycles, thereby dramatically reducing both manufacturing time and energy consumption.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The bacteria are applied to the loose aggregate in advance, and the cementation process begins immediately without requiring subsequent high-temperature firing or extended curing periods. The biochemical reactions proceed rapidly under ambient conditions, achieving structural integrity much faster than traditional methods that require days for firing and cooling.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach reduces atmospheric carbon dioxide emissions by producing a sustainable, low-embodied energy construction material that can be locally manufactured, using a process that mimics natural sandstone formation, and allows for the integration of additional performance traits like strength and insulation.

Implementation Method 1

Urease producing Sporosarcina Pasteurii, a nonpathogenic, common-soil bacterium has the ability to induce the production of calcite through a chemical reaction

Methodology Applied
Scientific EffectEnzyme catalysis: Enzyme

Implementation Method 2

the bacteria produce an enzyme, which in this case is urease, and use urea as a source of energy, producing ammonia and carbon dioxide

Methodology Applied
Scientific EffectChemical decomposition: Decomposition (biological)

Implementation Method 3

The rise in pH forms a mineral 'precipitate,' combining calcium chloride with carbon dioxide

Methodology Applied
Scientific EffectPrecipitation: Precipitation

Implementation Method 4

The calcium ions contribute to the formation of calcium carbonate. The calcium carbonate fills at least some of the gaps between the loose pieces of aggregate, bonding to the aggregate

Methodology Applied
Scientific EffectChemical reaction: Chemical Bonding

Implementation Method 5

The bacteria can then act as nucleation sites, attracting mineral ions from the calcium chloride to the cell wall, forming calcite crystals

Methodology Applied
Scientific EffectCrystallization: Crystallisation

Implementation Method 6

The hardened material is formed in a process referred to by Stocks-Fischer as microbial induced calcite precipitation [MICP]

Methodology Applied
Scientific EffectCementation: Chemical Bonding

Data Source

PatentEP3896047A1Methods for making construction material using enzyme producing bacteria
Publication Date: 2021.10.20 BIOMASON INC
  • EP3896047A1 patent drawingFigure 1
  • EP3896047A1 patent drawingFigure 2
  • EP3896047A1 patent drawingFigure 3

AI summary

Methods for producing construction material utilizing loose pieces of aggregate (30), enzyme producing bacteria, an amount of urea and an amount of calcium ions. A first solution is prepared which includes urease which is formed by enzyme producing bacteria. A second solution is prepared which includes urea and calcium ions. The first and second solutions are added to the loose aggregate (30). The calcium ions contribute to the formation of calcium carbonate wherein the calcium carbonate fills and bonds between at least some of the gaps between the loose pieces of aggregate forming a solid construction material (92).