Enzyme Culture Supplements for Cellular Agriculture Flavor and Texture
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Solution Overview
Problem
Cellular agriculture products often lack desirable attributes such as flavor, nutritional value, color, elasticity, and texture compared to traditional agriculture products, and existing additive techniques are insufficient or costly, and can mask undesirable attributes.
Innovation Solution
A culture supplement composition is used, comprising enzymes, substrates, and additives, to adjust the production of bioengineered cells, enhancing attributes like flavor, nutrition, color, and texture without reducing biomass production or cellular viability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If additives are applied to cells produced from cell cultures to improve flavor and other attributes, then the desirable attributes of the cellular agriculture product are improved, but the production complexity and cost increase
Solution Approach 1:
The patent applies culture supplements during the cell growth phase to pre-establish desirable molecular attributes (flavor, color, texture) before the cells are harvested and processed. This preliminary action eliminates the need for subsequent additive application steps, reducing production complexity while maintaining product quality
Solution Approach 2:
The patent modifies the cell culture medium parameters by incorporating specific culture supplements (amino acids, vitamins, minerals, growth factors) that change the molecular composition of the cells during growth. This parameter change approach allows direct control of product attributes at the source rather than requiring post-processing additives
2Manufacturing precision
If additives are applied to improve flavor and other attributes, then the desirable attributes are improved, but the production cost increases
Solution Approach 1:
By incorporating culture supplements during the cell growth phase, the patent establishes desirable product attributes through the cell's own metabolism rather than requiring expensive post-harvest additive application. This preliminary action reduces overall production costs by eliminating multiple processing steps
Solution Approach 2:
The culture supplements enable the cells to self-produce desirable molecular attributes (flavor compounds, pigments, textures) through their own metabolic pathways during growth, rather than requiring external additive application. This self-service approach reduces production costs by eliminating the need for separate additive formulation and application processes
3Manufacturing precision
If additives are used to improve product attributes, then flavor and other characteristics are enhanced, but the ingredient labels become more complicated
Solution Approach 1:
The patent incorporates all necessary molecular components directly into the cell culture medium during growth, so that the cells naturally produce the desired flavor and texture characteristics. This eliminates the need for separate additive ingredients in the final product, simplifying ingredient labels while maintaining quality
4Manufacturing precision
If additives are applied to mask undesirable attributes, then some flavor issues are addressed, but the undesirable attributes are not eliminated
Solution Approach 1:
The culture supplements are applied during the cell growth phase to pre-establish the correct molecular composition, including flavor compounds and structural elements. This preliminary action ensures that desirable attributes are built into the cells themselves rather than merely masked by additives, achieving more reliable and accurate attribute replication
Solution Approach 2:
The patent changes the fundamental molecular parameters of the cells during growth by providing specific culture supplements that alter cell metabolism. This produces cells with inherently improved molecular composition, achieving more reliable attribute replication rather than relying on masking additives
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The formulation aligns cellular agriculture products with consumer preferences by improving molecular attributes, reducing the need for additional additives and simplifying production processes.
Implementation Method 1
the formulation can include, in the carrier, at least one enzyme, at least one substrate, and at least one additive
Implementation Method 2
adjust production of a base cellular agriculture product to produce an adjusted cellular agriculture product
Implementation Method 3
at least one substrate... configured to adjust production of a base cellular agriculture product
Data Source
AI summary
Culture supplement compositions for cellular agriculture production include a formulation including, in a carrier, at least one enzyme, at least one substrate, and at least one additive. As embodied herein, the formulation is configured to adjust production of a base cellular agriculture product comprising bioengineered cells to produce an adjusted cellular agriculture product. Cell media for production of cellular agriculture products made using the compositions are also provided.