Enzyme Electrode Substrate Concentration Measurement Correction
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Solution Overview
Problem
Enzyme electrodes used for measuring substrate concentrations, such as glucose, suffer from variable sensitivity due to environmental changes and repeated use, leading to inaccurate measurements and the need for frequent calibration, which complicates reliability and accuracy.
Innovation Solution
A method and device that calculate substrate concentration using an output correction from an enzyme electrode, utilizing a reference solution with known substrate concentration, allowing for continuous monitoring and adjustment of sensitivity variations, enabling more accurate and reliable measurements by using a calculating unit to account for time-dependent changes in base outputs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If calibration is performed at fixed time intervals or startup, then the measurement process is simplified, but measurement accuracy deteriorates due to unaccounted sensitivity variations
Solution Approach 1:
The patent implements feedback by continuously monitoring the base output from the enzyme electrode and using this information to dynamically adjust calibration. The base output is sampled at multiple time points (T1, T2, T3) and used to calculate correction values that are applied to subsequent measurements. This closed-loop approach ensures that sensitivity variations are continuously compensated for, maintaining measurement accuracy without requiring complex manual calibration procedures.
Solution Approach 2:
The patent applies preliminary action by performing base output sampling and correction value calculation before actual substrate concentration measurements are taken. The base output is sampled at time points T1 (initially), T2 (after specimen supply), and T3 (after washing) to establish correction values in advance. This preliminary characterization of electrode behavior allows for accurate measurements to be performed subsequently without repeated manual calibration.
2Productivity
If enzyme electrode is used continuously, then productivity is improved, but reliability deteriorates due to sensitivity degradation
Solution Approach 1:
The patent enables continuous measurement by implementing an automated correction mechanism that operates throughout the measurement process. Instead of stopping for manual calibration, the system continuously samples the base output at predetermined time points (T1, T2, T3) and automatically calculates correction values. This allows the enzyme electrode to remain in continuous operation while the correction mechanism continuously compensates for sensitivity degradation, maintaining both productivity and reliability.
Solution Approach 2:
The patent uses feedback by continuously monitoring the base output and applying real-time corrections. The base output sampled at different time points provides feedback about electrode sensitivity changes, which are then used to calculate correction values applied to all subsequent measurements. This continuous feedback loop maintains measurement reliability throughout extended operation without interrupting productivity.
3Speed
If base output is sampled only at initial time point, then measurement speed is improved, but measurement accuracy deteriorates due to sensitivity changes
Solution Approach 1:
The patent performs preliminary base output sampling at multiple time points (T1, T2, T3) before and during the measurement process to establish comprehensive correction values. By sampling at T1 (initially), T2 (after specimen supply), and T3 (after washing), the system captures sensitivity variations throughout the measurement cycle. This preliminary action enables accurate substrate concentration calculations while maintaining efficient measurement speed through automated processing.
Solution Approach 2:
The patent applies dynamics by transitioning from static single-point base output sampling to dynamic multi-point sampling. Instead of taking base output only at the initial time point, the system dynamically samples at multiple time points (T1, T2, T3) corresponding to different stages of the measurement process. This dynamic approach captures sensitivity variations over time, enabling more accurate corrections without significantly impacting measurement speed due to automated processing.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach improves measurement accuracy and reliability by continuously monitoring and adjusting for sensitivity changes in enzyme electrodes, reducing the need for frequent calibration and stabilizing measurements even after electrode replacement.
Implementation Method 1
A conventionally known method called an electrode method, which is a method for measuring a concentration of a substrate such as glucose. According to the method, information correlating with a substrate concentration in a specimen is outputted to an electrode in contact with the specimen
Implementation Method 2
an enzyme electrode, which has an enzyme and a substrate selectively permeable film, is used
Implementation Method 3
an enzyme electrode, which has an enzyme and a substrate selectively permeable film
Implementation Method 4
A conventionally known method called an electrode method, which is a method for measuring a concentration of a substrate such as glucose
Data Source
AI summary
This invention provides a substrate concentration measuring method for measuring a concentration of a substrate included in a specimen based on an output for measurement from an enzyme electrode when the enzyme electrode and the substrate are reacted with each other, the substrate concentration is calculated using an output for correction from the enzyme electrode obtained when a reference solution whose substrate concentration is known and the enzyme electrode are reacted with each other before or after the enzyme electrode and the substrate are reacted with each other. For example, the output for correction is measured by each specimen. In this method, the substrate concentration may be calculated using the output for correction for the specimen to be measured and an output for correction corresponding to the at least one other specimen and measured prior to the output for correction.